8-氯腺苷抑制肿瘤坏死因子α诱导的视网膜细胞原癌基因表达的实验研究

目的 探讨8—氯腺苷(8-CLA)抑制肿瘤坏死因子α(TNF—α)诱导的视网膜色素上皮(RPE)细胞原癌基因的表达情况,为寻找抑制细胞增殖的新药提供依据。方法 取健康牛眼RPE细胞进行培养,采用Northern杂交法测定8-CLA对TNF—α诱导的RPE细胞c—fos及c—myc mRNA表达的影响。结果 正常培养的RPE细胞有少量c—fos及c—myc mRNA表达;加入TNF—α实验组,RPE细胞中c—fos及c-myc mRNA表达量增加,分别为对照组的3．1和1．5倍;加入TNF—α和8—CLA实验组,经TNF—α刺激的：RPE细胞中c—fos及c—myc mRNA表达量降低,分别较未用药组下降25．0％和29．0％。结论 8-CLA可抑制TNF—α诱导的RPE细胞c—fos及c—myc原癌基因的表达,此项研究结果将为临床探索有效抑制生长因子诱导的RPE细胞增殖的药物研究提供理论依据。

The inhibitory effect of 8-chloroadenosine on tumor necrosis factor-induced proto-oncogene expression of retinal pigment epithelial cells

OBJECTIVE: To investigate the inhibitory effect of 8-chloroadenodine (8-CLA) on tumor necrosis factor-alpha (TNF-alpha)-induced proto-oncogene expression in retinal pigment epithelial (RPE) cells. METHODS: Primary culture and subculture of health bovine RPE cells were established in vitro. RPE cells were treated with tumor necrosis factor TNF-alpha (13.3 mmol/L) with or without 8-CLA (16 micro mol/L) for 30 minutes or 3 hours, respectively. C-fos and c-myc mRNA expression in RPE cells was detected by Northern blot analysis. RESULTS: Normal RPE cells expressed c-fos and c-myc mRNA at a low level. After treatment with TNF-alpha, the expression of c-fos and c-myc mRNA was increased by 3.13 and 1.5 fold, respectively, as compared to untreated cells. 8-CLA decreased TNF-alpha-induced c-fos and c-myc mRNA expression by 25.0% and 29.0%, respectively. CONCLUSIONS: 8-CLA can inhibit TNF-alpha-induced c-fos and c-myc mRNA expression in RPE cells. These findings may be useful for exploring new drugs for inhibiting the growth-induced proliferation of RPE.