| HPLC法测定金氏肾炎丸中绿原酸含量 |
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| 引用本文： | 张海英,李伟,薛洁.HPLC法测定金氏肾炎丸中绿原酸含量[J].中国民族民间医药杂志,2010,19(17):9-10. |
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| 作者姓名： | 张海英 李伟 薛洁 |
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| 作者单位： | 新疆医科大学附属中医医院,新疆乌鲁木齐830000 |
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| 摘 要： | 目的：建立金氏肾炎丸（金银花、黄柏）中绿原酸的高效液相色谱含量测定方法。方法：样品用50％甲醇超声提取，过滤，取续滤液，采用RP—HPLC法测定。用Symmetry shield C18（3．9×150mm，5μm）分析柱，柱温：25℃；流动相：乙腈-0．4％磷酸溶液（10：90）；流速：1．0mL／min；检测波长：327nm；进样量10μL。结果：绿原酸进样量在8．0μg／mL～40．0μg／mL之间线性关系良好，r=0．9992（n=5），平均回收率为99．3％，RSD=1．41％（n=6）。结论：用该法测定金氏肾炎丸中绿原酸的含量，操作简单、重复性好、精密度高。
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| 关 键 词： | 金氏肾炎丸 绿原酸 高效液相色谱法 |
Detremination of Chlorogenic acid in Jinshishenyan Pills by HPLC |
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| Institution: | Zhang Hai - Ying, Li - Wei, Xue - Jie (ffilated Traditional Medical Hospital, Xinjiang Medical University; Urumqi 830000, China) |
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| Abstract: | Objective： To establish HPLC method for the determination of Chlorogenic acid in Jinshishenyan pills （Flos Lonicerae Japonicae, Cortex Phellodendri Chinensis）. Methods： The sample was extracted with 50% methanol through supersonic wave and then filtered. The determination was performed by HPLC on Symmetry shield Cls column （3.9 × 150mm, 5μm） , mobile phase was acetonitrile-0. 4% phosphoric acid （10： 90）, the dectection wavelength was 327nm, flow rate was 1.0mL/min and column temperature was 25℃. Result： There was good linearity in the range of 8. 0μg/mL -40. 0μg/mL, r =0. 9992 （n =5）; The average recovery of Chlorogenic acid was 99. 3%, RSD was 1.41% （n =6） o Conclusion： The method is simple, rapid, accurate and can be used for the quality control of Jinshishenyan pills. |
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| Keywords: | Jinshishenyan pills Chlorogenic acid HPLC |
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