25 Hydroxyvitamin D and vitamin E absorption in healthy children and children with chronic intrahepatic cholestasis

Patients with chronic cholestasis have reduced 25-hydroxyvitamin D (25 OHD) and vitamin E levels. We determined serum concentrations of 25 OHD, 1,25-dihydroxyvitamin D [1,25(OH)₂D] and vitamin E before and after oral administration of 10 g/kg body weight 25-hydroxyvitamin D₃ (25 OHD₃) and 100 IU/kg body weight vitamin E, respectively, in 4 patients with intrahepatic cholestasis and 6 healthy children. Vitamin E increased in all controls but in only one of the four patients. In contrast, oral 25 OHD₃ induced a normal rise in circulating 25 OHD and 1,25(OH)₂D. The low serum levels of 25 OHD in the patients before the oral bolus may have been due to inadequate parenteral vitamin D administration and/or to the simultaneous phenobarbital treatment. The latter possibility is supported by the increase of serum 25 OHD into the normal range after withdrawal of phenobarbital in one of the four patients.We conclude that vitamin E has to be supplemented parenterally or in water-soluble oral form. Further studies are necessary to clarify whether high-dose long-term oral 25 OHD₃ supplementation is sufficient to prevent vitamin D deficiency in patients with chronic cholestasis.Abbreviations 25 OHD 25-hydroxyvitamin D - 25 OHD₃ 25-hydroxyvitamin D₃ - 24,25(OH)₂D 24,25-dihydroxyvitamin D - DBP vitamin D binding proteinThis report was presented in part at the XIX European Symposium on Calcified Tissues in Stockholm, June 1986. This study was supported by the Deutsche Forschungsgemeinschaft Bu-199-9-1