| 单壁碳纳米管对血管外膜成纤维细胞的毒性作用 |
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| 引用本文： | 刘丽华,林治卿,张华山,杨红莲,刘焕亮,黄洁华,张伟,袭著革.单壁碳纳米管对血管外膜成纤维细胞的毒性作用[J].解放军预防医学杂志,2011,29(3):160-163. |
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| 作者姓名： | 刘丽华 林治卿 张华山 杨红莲 刘焕亮 黄洁华 张伟 袭著革 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所,天津市环境与食品安全风险监控技术重点实验室,天津300050 |
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| 基金项目： | 国家自然科学基金课题(No.20877102)国家自然科学基金课题(No.20907075) |
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| 摘 要： | 目的 探讨不同剂量单壁碳纳米管(SWCNTs)对原代培养大鼠血管外膜成纤维细胞(VAF)的毒性作用,为SWCNTs的毒理学及生物安全性研究提供参考.方法 将SWCNTs制成颗粒悬液,设9个剂量组(0.80、1.60、3.20、6.25、12.50、25.00、50.00、100.00、200.00μg/ml)对原代培养...
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| 关 键 词： | 单壁碳纳米管 血管外膜成纤维细胞 细胞毒性 氧化应激 |
CYTOTOXICITY OF SINGLE WALL CARBON NANOTUBES ON RAT VASCULAR ADVENTITIAL FIBROBLASTS |
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| LIU Li-hua,LIN Zhi-qing,ZHANG Hua-shan,YANG Hong-lian,LIU Huan-liang,HUANG Jie-hua,ZHANG Wei,XI Zhu-ge.CYTOTOXICITY OF SINGLE WALL CARBON NANOTUBES ON RAT VASCULAR ADVENTITIAL FIBROBLASTS[J].Journal of Preventive Medicine of Chinese People's Liberation Army,2011,29(3):160-163. |
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| Authors: | LIU Li-hua LIN Zhi-qing ZHANG Hua-shan YANG Hong-lian LIU Huan-liang HUANG Jie-hua ZHANG Wei XI Zhu-ge |
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| Institution: | LIU Li-hua,LIN Zhi-qing,ZHANG Hua-shan,YANG Hong-lian,LIU Huan-liang,HUANG Jie-hua,ZHANG Wei,XI Zhu-ge(Key Laboratory of Environmental and Food Safety Risk Monitoring Technology,Institute of Hygiene and Environmental Medicine,Academy of Military Medical Sciences,Tianjin 300050,China) |
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| Abstract: | Objective To explore the toxic effect of single wall carbon nanotubes（SWCNTs） at different doses on vascular adventitial fibroblasts（VAF）,and provide the basis for toxicological and biological safety study of SWCNTs.Methods Primary VAF cells were exposed to SWCNTs suspension at nine different concentrations（0.80,1.60,3.20,6.25,12.50,25.00,50.00,100.00,200.00 μg/ml） for 24 h.MTT and WST-1 assay were used to evaluate the cell viability.Then VAF cells were exposed to SWCNTs suspension for 48 h at five different concentrations（0.80,3.20,12.50,50.00,200.00 μg/ml） based on the IC50 found.MDA and SOD were used to test the degree of oxidative damage.Results MTT showed that the cell viability was 41.0% at a dose of 100 μg/ml,and different from that of control（72.3%）,（P0.05）;WST showed that the cell viability was 51.2% at a dose of 50 μg/ml,different from that of control（77.2%）,（P0.05）.The activity of SOD（9.61 U/mg prot） at a dose of 12.50 μg/ml was lower than that of control（14.24 U/mg prot）,（P0.05）.The content of MDA（0.30 nmol/mg prot） at 3.20 μg/ml was also lower than that of control（0.23 nmol/mg prot）,（P0.05）.Conclusion SWCNTs suspension of certain concentration could decrease VAF cell viability,and induce oxidative stress,thus result in toxicity to the cell. |
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| Keywords: | single wall carbon nanotube rat vascular adventitial fibroblasts cytotoxicity oxidative stress |
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