脑复聪提高脑缺血小鼠记忆能力的机理研究

目的：探讨自拟中药方脑复聪颗粒对脑缺血小鼠记忆能力及大脑胆碱能系统的影响。方法：采用双颈总动脉闭塞-缺血-再灌术造成学习记忆障碍小鼠模型。小鼠记忆能力以Morris圆形水迷宫的潜伏期作为指标。结果：小鼠脑缺血后7天，其潜伏期[(82．3±32．0)s]和游程[(986．7±414．5)cm]比假手术组[(35．3±37．9)s，(410．4±507．0)cm]延长(P<0．01)；脑复聪治疗后[(30．6±31．3)s，(378．1±386．3)cm]恢复正常(P<0．01)。大脑皮层和海马的胆碱乙酰化转移酶活性[(35．64±4．96)pmol／(mgpro·min)，(45．08±4．96)pmol／(mgpro·min)]比假手术组[(40．52±4．74)pmol／(mgpro·min)，(53．96±8．53)pmol／(mgpro·min)]降低、大脑皮层胆碱能M受体活性[(44．41±10．67)nmol／mgpro]比假手术组[(57．56±6．98)nmo1／mgpro]降低、大脑皮层和海马的胆碱能N受体活性[(232．41±21．99)pmol／mgpro，(303．72±28．78)pmo1／mgpro]比假手术组[(276．66±32．46)pmol／mgpro，(385．56±46．88)pmol／mgpro]降低(P<0．05．P<0．01)，脑复聪组及当归芍药散组提高到正常水平(P<0．05，P<0．01)。结论：脑复聪颗粒对脑缺血所致的智能障碍有保护作用，其机理可能与提高胆碱能系统功能有关。

Study on the Mechanism of Naofucong Granule in Improving Memory of Cerebral Ischemic Mice

OBJECTIVE: To explore the effect of Naofucong Granule (NFCG), a self-manufactured Chinese herbal preparation, on memory and cerebral cholinergic system in cerebral ischemic mice. METHODS: Mice model of learning/memory impairment was established by bilateral ligation of internal carotid arteries. The memory ability of mice was assessed by measuring latent period in the Morris Water Maze. RESULTS: Seven days after modeling, the latent period and swimming course of mice (82.3 +/- 32.0 seconds and 986.7 +/- 414.5 cm) were longer than those of control (sham-operated mice, 35.3 +/- 37.9 seconds and 410.4 +/- 507.0 cm, P < 0.01). Administration of NFCG could ameliorate these changes to 30.6 +/- 31.3 seconds and 378.1 +/- 386.3 cm (P < 0.01) and normalized. In the model animals, the choline acetylase activity in brain cortex and hippocampus was 35.64 +/- 4.96 and 45.08 +/- 4.96 respectively, which was significantly lower than that in the control (40.52 +/- 4.74 and 53.96 +/- 8.53); so was the activity of cholinergic receptor M in cerebral cortex (44.41 +/- 10.67 vs 57.56 +/- 6.98) and the activity of cholinergic receptor N in cerebral cortex and hippocampus (232.41 +/- 21.99 and 303.72 +/- 72 +/- 28.78 vs 276.66 +/- 32.46 and 385.56 +/- 46.88), P < 0.05 or P < 0.01. Administration of NFCG also could reverse them and elevate to normal level. CONCLUSION: NFCG has protective function on intellectual deficits in cerebral ischemic mice, which may be related to its action in enhancing function of cholinergic system.