Wy14643对急性肺损伤大鼠肺组织抗炎作用的实验研究

目的探讨Wy14643对急性肺损伤（Acutelung injury，ALI）大鼠肺组织的保护作用及其可能机制。方法将128只雄性Wistar大鼠随机等分为对照组、致伤组、Lw 1 mg组、LW 3 mg组。用LPS（5me／kg）静脉注射复制大鼠Au模型，在静脉注射LPS 15 min后再次静脉注射Wy146431mg／kg和3mg／kg，分别在LPS后1、2、4及8h时活杀大鼠，分别采用酶联免疫吸附分析法及分光光度计法检测用药前、后各时相点大鼠肺组织匀浆及血浆中TNF—α、IL-10浓度及MPO活性。结果LW 1 mg组在2、4及8h，LW 3 mg组在1、2、4及8h血浆及肺组织匀浆上清中的TNF—α、IL-10分别较Au组相应时相点显著下降与升高（P〈0．05）；LW 1 mg组在4及8h，LW3mg组在2、4及8h肺组织匀浆上清中的MPO活性均较ALI组相应时相点显著下降（P〈0．05）。结论Wy14643对Au大鼠肺组织具有-定的保护作用，其机制可能是PPARa激活后抑制了TNF-α的释放，增加IL-10的释放，减轻肺部及全身的炎症反应。

The antiinflammatory of WY14643 in acute lung injury in rats

Objective To investigate the effect of WY14643 on acute lung injury and its mechanism. Methods 128 male Wistar rats were randomly divided into four groups, including control group, acute lung injury group, LW 1 mg group and LW 3 mg group. Wistar rats were treated with lipopolysaccharide before intratracheal challenge with Wy14643. All the groups were killed at 1, 2, 4 and 8 h after LPS injection. TNF-α and IL-10 in lung were measured with ELISA. Results The level of TNF-α and IL-10 in supematant liquid of rats lung homogenate and in hematoplasma at 2, 4 and 8h in LW 1 mg group LW 3 mg group were significandy lower and higher than those in ALl group（ P 〈0.05 ）. The level of MPO in ALl rats were significantly decreased. Conclusions The results suggest that Wy14643 may be useful to treat acute lung injury, whose mechanisms may involve that Wyi4643 decreases the expression of TNF-α and increases the expression of IL-10.