| 高通量检测技术检测乙型肝炎病毒变异的临床意义 |
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| 引用本文： | 刘伟,陈双峰,王凤菊,刘义华.高通量检测技术检测乙型肝炎病毒变异的临床意义[J].上海医学检验杂志,2006(5). |
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| 作者姓名： | 刘伟 陈双峰 王凤菊 刘义华 |
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| 作者单位： | 聊城市人民医院中心实验室 山东聊城252000 |
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| 摘 要： | 目的探讨基因芯片技术检测乙型肝炎病毒(HBV)6位点变异的临床意义。方法对91例乙型肝炎患者采用基因芯片技术,检测HBV 6位点的自然变异。结果91例乙型肝炎患者HBV变异率98.9%(90/91),其中BCP区1762位点变异率最高(61.5%)。重度肝炎和肝硬化中双位点变异显著高于轻中度肝炎(P<0.01)。29例进行过拉米呋啶抗病毒治疗的病例中P区552位点变异率为96.6%(28/29)。结论基因芯片技术检测HBV变异具有高通量、高灵敏等特点,对观察HBV基因突变有很高的临床应用价值。
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| 关 键 词： | 基因芯片 乙型肝炎病毒 变异 |
Detection of hepatitis B virus gene mutations by high-throughput method and its clinical significance |
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| LIU Wei,CHEN Shuangfeng,WANG Fengju,LIU Yihua.Central Laboratory,Liaocheng People's Hospital,Shandong Liaocheng ,China.Detection of hepatitis B virus gene mutations by high-throughput method and its clinical significance[J].Shanghai Journal of Medical Laboratory Sciences,2006(5). |
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| Authors: | LIU Wei CHEN Shuangfeng WANG Fengju LIU YihuaCentral Laboratory Liaocheng People's Hospital Shandong Liaocheng China |
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| Institution: | LIU Wei,CHEN Shuangfeng,WANG Fengju,LIU Yihua.Central Laboratory,Liaocheng People's Hospital,Shandong Liaocheng 252000,China |
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| Abstract: | Objective To study the clinical significance of the detection of hepatitis B virus(HBV) gene mutations by gene chip technique. Methods 6 sites mutations of HBV in 91 patients were detected by gene chip technique. Results The mutations of HBV existed in all the 91 patients except one(90/91),the rate of mutation was 98.9%.The mutation rate of BCP section 1762 site was the highest(61.5%).Double sites mutations in the patients of severe hepatitis and cirrhosis were significanly higher than in mild hepatitis(P<0.01).The mutation rates of P section 552 site were 96.6%(28/29) in 29 patients treated by lamivudine. Conclusions The gene chip technique is simple to perform and can be used as a rapid and high-throughput method.It has a high clinical value in detecting the HBV gene mutation. |
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| Keywords: | Gene chip Hepatitis B virus Mutation |
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