| 福建省新分离3株乙型脑炎病毒的分子特征鉴定 |
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| 引用本文： | 陈端,王环宇,付士红,宋宏,邓娟,杨益良,梁国栋.福建省新分离3株乙型脑炎病毒的分子特征鉴定[J].中华实验和临床病毒学杂志,2005,19(1):5-8. |
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| 作者姓名： | 陈端 王环宇 付士红 宋宏 邓娟 杨益良 梁国栋 |
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| 作者单位： | 1. 350001,福州,福建省疾病预防控制中心病毒室
2. 中国疾病预防控制中心病毒病预防控制所病毒基因工程国家重点实验室 |
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| 基金项目： | 国家自然科学基金资助(30170046) |
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| 摘 要： | 目的 确定福建省新分离乙型脑炎病毒 (JEV)的基因分型及其E区段氨基酸序列特征。方法 用逆转录聚合酶链反应 (RT PCR)扩增并克隆新分离JEV(0 2 4 1、0 2 4 3、0 2 10 2 )的PrM、E区段核苷酸序列 ,测序后应用ClustalX软件做碱基配对和比较分析 ,种系发生采用PHYLIP软件包分析。结果 新分离的 3株JEV属于基因Ⅲ型 ,E区段核苷酸和氨基酸与减毒活疫苗株SA 14 14 2株的同源性均在 96 %以上 ,在关键结构域有部分氨基酸差异。结论 福建省新分离的 3株病毒属于基因Ⅲ型JEV ,E蛋白与疫苗株相比有部分氨基酸差异
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| 关 键 词： | 脑炎病毒 日本 碱基序列 基因型 种系发生 |
| 修稿时间： | 2004年3月29日 |
Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province |
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| Chen Duan,WANG Huan-yu,FU Shi-hong,SONG Hong,Deng Juan,YANG Yi-liang,LIANG Guo-dong.Molecular characteristics of three new isolates of Japanese encephalitis virus in Fujian Province[J].Chinese Journal of Experimental and Clinical Virology,2005,19(1):5-8. |
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| Authors: | Chen Duan WANG Huan-yu FU Shi-hong SONG Hong Deng Juan YANG Yi-liang LIANG Guo-dong |
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| Institution: | State Key Laboratory for Molecular Virology, Fujian Center for Disease Control and Prevention, Fuzhou 350001, China. |
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| Abstract: | OBJECTIVE: To analyze the genotype of newly isolated Japanese encephalitis viruses in Fujian province and the characteristics of amino acid sequence in the E gene. METHODS: PrM and E segments of newly isolated Japanese encephalitis viruses were amplified by RT-PCR, the PCR products were cloned into T vector for sequencing. Phylogenetic analysis was carried out by PHYLIP program. RESULTS: Newly isolated Japanese encephalitis viruses belonged to genotype III, the nucleotide and amino acid of E gene showed high homology to vaccine strain SA14-14-2, but had some difference in some domains. CONCLUSION: Newly isolated viruses in Fujian province belonged to Japanese encephalitis virus genotype III. E protein of the isolates showed some differences as compared with vaccine strains. |
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| Keywords: | Encephalitis virus Japanese Base sequence Genotype Phylogeny |
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