| 正交试验优化提取黄连中小檗碱型生物碱 |
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| 引用本文： | 应懿,何志红,周世文,汤建林,黄永平,杨雪.正交试验优化提取黄连中小檗碱型生物碱[J].中国药房,2007,18(9):670-672. |
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| 作者姓名： | 应懿 何志红 周世文 汤建林 黄永平 杨雪 |
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| 作者单位： | 第三军医大学附属新桥医院临床药理基地,重庆市,400037 |
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| 基金项目： | 致谢：感谢四川大学华西药学院生药教研室张浩教授、陈雏老师对试验的无私帮助! |
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| 摘 要： | 目的:研究黄连中小檗碱型生物碱的最佳提取工艺。方法:采用正交试验设计,以小檗碱、巴马汀、黄连碱、表小檗碱和药根碱的总含量为考察指标,考察乙醇浓度、提取时间、提取次数、乙醇用量4个因素对提取结果的影响。结果:最佳提取工艺为8倍量的50%乙醇,回流提取4次(每次2h)。结论:本工艺重现性好,测定方法可行,为大批量黄连提取生物碱提供了理论依据。
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| 关 键 词： | 黄连 生物碱 正交试验 |
| 文章编号： | 1001-0408(2007)09-0670-03 |
| 收稿时间： | 10 24 2006 12:00AM |
| 修稿时间： | 12 24 2006 12:00AM |
Optimizing the Extraction of Alkaloids from Rhizoma Coptidis by Orthogonal Design |
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| YING Yi,HE Zhihong,ZHOU Shiwen,TANG Jianlin,HUANG Yongping,YANG Xue.Optimizing the Extraction of Alkaloids from Rhizoma Coptidis by Orthogonal Design[J].China Pharmacy,2007,18(9):670-672. |
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| Authors: | YING Yi HE Zhihong ZHOU Shiwen TANG Jianlin HUANG Yongping YANG Xue |
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| Institution: | Clinical Pharmacology Base of Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China |
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| Abstract: | OBJECTIVE:To study the optimized process for extraction of the alkaloids from Rhizoma Coptidis.METHODS:Orthogonal design was used for studying the effect of alcohol concentration,volume,extraction time and times on the content of jatrorrhizine,epiberberine,coptisine,palmatine and berberine.RESULTS:The optimum extraction process is as follows:8 times of 50% alcohol,extracted for 4 times,each time for 2 hours.CONCLUSION:This extraction process is workable and reproducible,and can provide theoretic support for mass extraction of the alkaloids from Rhizoma Coptidis. |
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| Keywords: | Rhizoma Coptidis Alkaloids Orthogonal test |
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