Abstract: | Objectives: Creating polymerase chain reaction(PCR)
technique to detect Mycoplasma genitalium(Mg) infection rates in sexually transmitted
disease(STD) high risk populations from different geographical areas in our country,discussing
the role of Mg in STD. Methods: The urogenital specimens from 423 cases of STD
department,261 sexually promiscuous population and 73 normal persons,who were from
Nanjing,Shanghai,Guangdong and Kunmin,were detected for MgDNA by polymerase chain
reaction technique. Results: MgDNAPCR positive rate in control group was 1.79%,which was
significantly lower than that(15.1%) in group of STD department and that(8.81%) in group of
promiscuous population(P<0.01),respectively.Otherwhise,there was a remarkable difference of
MgDNAPCR positive rate between groups of STD department and promiscuous population.Mg
infection rate of STD population in Guangdong and Kunmin was much higher than that of
Shanghai,Nanjing(P<0.05) and not differences was found between Shanghai and Nanjing,also
Gua dong and Kunmin.There were not differences between group without NGH,UU,CT infection
and groups with NGH,UU,CT infection alone or commonly(P>0.05). Conclusions: Mg infection
rate was higher in STD highrisk populations(including STD patients and promiscuous persons)
than that in normal group in China.Higher MgDNA positive rate was found in Guangdong and
Kunmin,where there was a higher STD occurance.There was a same MgDNA positive rate in the
specimens with or without other microorganism infection.Mg might also be an important
microorganism of STD,which is helpful for Mg epidemiologic observation and controlling the
transmission of STD. |