Molecular cloning of the 3-phosphoglycerate kinase (PGK) gene from Aspergillus nidulans |
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Authors: | John M Clements Clive F Roberts |
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Institution: | (1) Department of Genetics, School of Biological Sciences, University of Leicester, LE1 7RH Leicester, UK |
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Abstract: | Summary The Aspergillus nidulans 3-phosphoglycerate kinase gene (PGK) has been isolated from a phage genomic library, using the equivalent yeast gene as a hybridization probe. The location of the PGK gene within the cloned DNA has been physically mapped. The DNA sequence of a small region of the putative PGK has been determined and found to code for amino acids corresponding to the N-terminal end of the PGK protein. In contrast to the yeast PGK gene the Aspergillus gene contains a 57 base pair intron occurring between the coding sequences for amino acid 22 and 23.A DNA fragment encompassing the PGK gene was shown to hybridize a 1,700 base poly(A) mRNA, sufficient to encode the PGK polypeptide. |
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Keywords: | Aspergillus Cloning PGK mRNA |
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