血管钠肽抑制低氧刺激心成纤维细胞c-fos的表达

目的　研究血管钠肽 (VNP)对低氧作用时心成纤维细胞增殖的影响 ,并对其机制进行探讨 .方法　分离纯化乳鼠心成纤维细胞 ,随机分为 4组 :对照组、低氧组 (2 0～ 30m L· L- 1 )、VNP组 (10 - 8～ 10 - 6 mol· L- 1 )和 VNP (10 - 6mol· L- 1 ) +低氧组 .以免疫组织化学染色方法观察各组 c-Fos的表达情况 ,采用激光共聚焦方法测定了 Ca2 + ]i 水平 .结果　 1低氧组较对照组可以显著升高 Fos样免疫阳性细胞数及染色强度 (P<0 .0 5 ) ;2 VNP预处理心成纤维细胞可以减弱低氧的作用 (P<0 .0 5 vs低氧组 ) ,但 Fos的表达量仍高于对照组 (P<0 .0 5 ) ;3对照组 Ca2 + ]i 水平无显著变化 ,低氧组 Ca2 + ]i 水平显著升高 ,而 VNP能使升高的 Ca2 + ]i 水平较对照组和低氧组显著降低 (P<0 .0 5 ) .结论　 VNP能减弱低氧对心成纤维细胞生长的刺激作用 ,其机制可能与 Ca2 + 等信号转导分子及 c- fos表达有关

Vasonatrin peptide attenuates hypoxia-induced expression of c-Fos gene in cultured cardiac fibroblasts

Chief aim of the present work was to investigate vasonatrinpeptide (VNP) attenuates hypoxia-induced expression of c-fos gene in cardiac fibroblasts from cultured neonatal rats. The cultured cardiac fibroblasts were divided randomly into four groups: Control group, hyoxia group (20～30 mL·L-1), VNP group (10-8～10-6 mol·L-1) and VNP (10-6 mol·L-1)+hypoxia group. The c-Fos expression of cardiac fibroblasts was measured by the means of immunocytochemistry, Ca2+]i concentration was measured by the means of interactive laser cytometer. The studies performed with these techniques show that hypoxia increased significantly the positive cells and mean grey of cardiac fibroblasts (P<0.05 vs control group), VNP decreased the positive cells (P<0.05 vs control group) and decreased the level of Ca2+]i in VNP+hypoxia group. These findings indicate that VNP can attenuate hypoxia-induced expression of c-fos gene in cardiac fibroblasts which was associated with the changes of theCa2+]i.