耐药性结核分枝杆菌的分子生物学检测方法研究进展

结核病的重新流行使人类健康再次面临严重威胁,为提高抗结核药物的治疗效果,临床需要对结核分枝杆菌的耐药性进行快速鉴定。本文阐述了基于DNA测序技术(DNA直接测序、焦磷酸测序技术)、DNA扩增技术(包括巢式 PCR、PCR-SSCP 法、多位点酶切联合PCR-SSCP法、异源双链形成法、分子信标法等)、分子杂技术(包括限制性片段长度多态性(RFLP)分析、多列探针检测法(LiPA)、基因芯片技术)和 DNA 信号放大技术等分子生物学检测方法在检测结核分枝杆菌耐药性方面的研究进展,以期为临床提供一定的方法参考。

Recent Advances in Research on Molecular Detection for Resistance of Mycobacterium Tuberculosis

Tuberculosis, the infectious disease, is prevalent again and it threatens the health of human being. In order to increase the therapeutic effect of anti-tuberculosis drugs, it is necessary to make rapid detection for resistance of Mycobacterium tuberculosis. In this review, it introduced the new progress in molecu- lar detection methods, which are mainly based on DNA sequencing(such as direct DNA sequencing, pyrosequen- cin),polymerase chain reaction(PCR)including nest PCR, polymerase chain reaction single stranded conforma- tional polymorphism(PCR-SSCP), PCR-SSCP digesting with restriction endonuclease, heteroduplex formation and molecular beacon) and gene blotting, including restriction fragmant length polymorphism(RFLP), line probe assay(LiPA), DNA chips and DNA signal amplification technique, separately, for resistance of Mycobacterium tuberculosis, which may be helpful to the clinic of treatment for tuberculosis.