HPLC法测定野生与栽培肿节风中迷迭香酸的含量

目的建立野生与栽培肿节风中迷迭香酸含量的测定方法,分析比较肿节风不同部位中迷迭香酸含量的差异。方法采用HPLC法,固定相为Phenomenex C18色谱柱(250mm×4.6mm,4μm);流动相为乙腈-1mL.L-1磷酸溶液(25∶75);流速为1.0mL.min-1,柱温为40℃,检测波长为342nm。结果野生肿节风根、茎、叶中迷迭香酸的平均含量分别为0.25%,0.42%和1.43%,栽培肿节风根、茎、叶中迷迭香酸的平均含量分别为0.29%,0.49%和1.50%。结论肿节风药材根、茎、叶3个部位中的迷迭香酸含量差异较大,叶中含量最高,茎中其次,根中最低。相同部位比较,栽培肿节风较野生的迷迭香酸含量高。

Determination of romarinic acid in wild and cultivated Sarcandra glabra by HPLC

Objective To determine the content of romarinic acid in the roots,stems and leaves of wild and cultivated Sarcandra glabraby HPLC method.Method Phenomenex C18column(250mm×4.6mm,4μm)was used,the mobile phase was acetonitrile1mL.L-1 phosphoric acid(25∶75),and the flow rate was 1.0mL.min-1.The column temperature was set at 40℃,the detection wavelength was 342nm,and the injection volume was 5μL.Result The average content of rosmarinic acid in roots,stems and leaves of the wild was 0.25%,0.42%and 1.43%,respectively,and average content of rosmarinic acid in the cultivated was 0.29%,0.49%and 1.50%,respectively.Conclusion The content of rosmarinic acid is significantly different in roots,stems and leaves.The cultivated showed a higher content of rosmarinic acid than the wild in the same organ.