电针对慢性应激抑郁模型大鼠海马神经元凋亡与再生的影响

目的:观察电针对慢性应激抑郁模型大鼠海马组织中抗凋亡因子(Bcl-2)和生长相关蛋白-43(GAP-43)表达的影响,探讨电针对慢性应激抑郁大鼠海马神经元凋亡与再生的影响机制。方法:32只SD大鼠,随机分组为空白组、模型组、电针组、氟西汀组。采用旷场实验进行行为学评价;采用免疫组化法检测海马组织中Bcl-2和GAP-43的表达。结果:模型组大鼠旷场试验水平穿越格数竖立次数次均明显低于空白组(P0.05);电针组、氟西汀组均明显高于模型组(P0.05)。模型组与空白组之间海马中Bcl-2表达没有显著性差异;电针组与模型组之间有显著性差异(P0.05)。模型组与空白组比较海马中GAP-43表达显著降低(P0.05);氟西汀组与模型组相比表达均有增高趋势,电针组的表达则显著高于模型组(P0.05)。结论:慢性应激可以引起大鼠的活动量降低和探究行为减少,电针可能通过调节海马神经元凋亡与再生而改善慢性应激抑郁大鼠的行为学症状,可能是电针抗抑郁效应的作用机制之一。

Effect of hippocampal neurons apoptosis and regeneration on chronic stress depression model rat treated by Electroacupuncture

Objective: To explore the mechanism of electroacupuncture on chronic stress depression,we observed the expression of Bcl-2 and GAP-43 in hippocampus of stress rats.Methods: Tirthty-two Sprague-Dawley rats were randomly divided into control group,model group,electroacupuncture group and Prozac group.Open-filed test was used to detect the behavior of rats.Immunohistochemistry method was used to detect the expression Bcl-2 and GAP-43 in hippocampus.Results: After stress exposure,compared with control group,the crossing numbers of model group were significantly less than those of control group(P<0.05),the electroacupuncture group and Prozac group were significantly increased(P<0.05).The expression of Bcl-2 in hippocampus of model group showed no difference from control group.Hippocampal Bcl-2 expression in electroacupuncture group was higher than model group(P<0.05).Compared with the control group,GAP-43 expression in hippocampus of model group was lower(P<0.05).GAP-43 expression in hippocampus of electroacupuncture group,Pozac group were higher than model group,and the GAP-43expression in the electroacupuncture group were significant different from model group(P<0.05).Conclusion: Chronic stress could cause the rats’ activities decreased and exploratory behavior reduced,while electroacupuncture could improve the behavior of stress rats by decreasing the hippocampal apoptosis,which maybe one of the mechanisms of antidepressant effect of electroacupuncture.