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| 蛋白激酶C-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响 | |
| 引用本文： | 雷少青,高霞,刘慧敏,徐波,徐金金,夏中元,夏正远. 蛋白激酶C-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响[J]. 武汉大学学报(医学版), 2011, 32(2): 141-145,封2 |
| 作者姓名： | 雷少青 高霞 刘慧敏 徐波 徐金金 夏中元 夏正远 |
| 作者单位： | 1. 武汉大学医学院药理学教研室,湖北,武汉,430071;武汉大学人民医院麻醉科,湖北,武汉,430060 2. 首都医科大学附属北京朝阳医院内分泌科,北京,100020 3. 武汉大学人民医院麻醉科,湖北,武汉,430060 4. 首都医科大学附属北京友谊医院内科,北京,100050 |
| 基金项目： | 国家自然科学基金资助项目(编号:30872447) |
| 摘 要： | 目的:探讨白蛋白激酶c-β抑制剂LY333531对早期糖尿病大鼠心脏抗氧化状态及NADPH氧化酶的影响.方法:24只SD雄性大鼠(240-260 g),随机分为正常对照组(NC)、糖尿病组(DM)及糖尿病LY333531治疗组(LY).4周后试剂盒检测血浆及心肌组织中的15-F2t-Isoprostane与总抗氧化浓度... |
| 关 键 词： | LY333531 糖尿病心肌病 NADPH氧化酶 氧化应激 |
Effects of LY333531 on Antioxidant State and Myocardial NADPH Oxidase in Early Diabetic Rats |
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| LEI Shaoqing,GAO Xia,LIU Huimin,XU Bo,XU Jinjin,XIA Zhongyuan,XIA Zhengyuan. Effects of LY333531 on Antioxidant State and Myocardial NADPH Oxidase in Early Diabetic Rats[J]. Medical Journal of Wuhan University, 2011, 32(2): 141-145,封2 | |
| Authors: | LEI Shaoqing GAO Xia LIU Huimin XU Bo XU Jinjin XIA Zhongyuan XIA Zhengyuan |
| Affiliation: | LEI Shaoqing1,3,GAO Xia2,LIU Huimin3,XU Bo4,XU Jinjin3,XIA Zhongyuan3,XIA Zhengyuan1,31Dept.of Pharmacology,School of Medicine,Wuhan University,Wuhan 430071,China2Dept.of Endocrinology,Affiliated Beijing Chaoyang Hospital of Capital Medical University,Beijing 100020,China3Dept.of Anesthesiology,Renmin Hospital of Wuhan University,Wuhan 430060,China4Dept.of Internal Medicine,Affiliated Beijing Friendship Hospital of Capital Medical University,Beijing 100050,China |
| Abstract: | Objective: To explore the effects of PKC-β inhibitor LY333531 on myocardial NADPH oxidase in early diabetic rats.Methods: Twenty-four male SD rats were randomly assigned to control group(NC),diabetic group(DM) and diabetic group with LY333531(LY) treatment for four weeks.The levels of 15-F2t-Isoprostane and total antioxidant concentration in plasma and heart tissues were assessed by Reagent kit.Morphological changes of cardiomyocytes were observed under light microscope with hematoxylin-eosin staining.The subunits P22phox,P67phox and gP91phox of NADPH oxidase were analyzed by Western blot.Results: The structure of cardiac tissues in DM group was disordered,LY treatment made it close to normal.The 15-F2t-Isoprostane levels and total antioxidant concentrations in plasma and heart tissues,the protein expression of P22phoxand gP91phox,and the membrane translocation of P67phox were significantly increased in diabetic group as compared to control group(all P<0.05).LY treatment decreased the levels of 15-F2t-Isoprostane in plasma and heart tissues,as well as plasma total antioxidant concentration,P22phox expression,and membrane translocation of P67phox as compared to control group(P<0.05),but there was not a significant change in gP91phox expression.Conclusion: The LY333531 may protect diabetic myocardium from oxidative injury through inhibiting NADPH oxidase activation and expression. |
| Keywords: | LY333531 Diabetic Cardiomyopathy NADPH Oxidase Oxidative Stress |
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