p73及p53基因多态性与甘肃武威人群胃癌风险相关性研究

目的]评价p73基因G4C14-to-A4T14双核苷酸多态性（p73 G4A DNP）和p53基因第72密码子单核苷酸多态性（p53 Arg72Pro SNP）与甘肃武威市人群胃癌高发风险及胃癌不同病理亚型的相关性。方法]p73G4ADNP的基因分型采用两双相对引物多聚酶链式反应法,p53 Arg72Pro SNP基因分型采用PCR-RFLP法。结果]共检查胃癌病例385例以及健康对照412人。胃癌组中弥漫型胃癌305例（79.22%）,肠型胃癌80例（20.78%）。对照组p73AT/AT、AT/GC及GC/GC基因型的频率分别为28.15%、47.09%和24.76%;胃癌组分别为21.98%、45.04%和32.98%;以AT/AT作为指示物,胃癌组和弥漫型胃癌的GC/GC纯合子基因型频率均高于对照组,优势比（OR）分别为1.71（95%CI,1.16～2.51）和1.87（1.24～2.81）。对照组p53基因Pro/Pro、Pro/Arg以及Arg/Arg基因型的频率分别为27.18%、50.49%及22.33%;胃癌组则分别为21.82%、45.45%和32.73%;以Pro/Pro为指示物,胃癌组和弥漫型胃癌Arg/Arg基因型频率显著高于对照组,OR分别为1.83（95%CI,1.24～2.70）和2.25（95%CI,1.47～3.43）。结论]携带p73 G4A GC/GC基因型或p53 Arg/Arg基因型可能会增加胃癌,尤其是弥漫性胃癌的发病风险。

Association of Genetic Polymorphisms of p73 Gene and p53 Gene with the Risk of Gastric Cancer in Wuwei Population

Purpose] To evaluate the associations between p73 gene G4C14-to-A4T14,p53 Arg72Pro SNP and gastric cancer risk in Wuwei City,Gansu Province.Methods] The genotyping of p73 gene G4A DNP was conducted by the polymerase chain reaction with the confronting two-pair primers(PCR-CTPP).The genotyping of p53 codon 72 SNP was performed using PCR-RFLP.Results] A total of 385 cases with gastric cancer and 412 healthy controls were investigated.There were 305(79.22%) diffuse-type cases and 80(20.78%) intestinal-type ...