高效液相色谱法测定潜朴中厚朴酚及和厚朴酚的含量

目的建立潜朴药材中厚朴酚（magnol01）及和厚朴酚（honoki01）的含量测定方法。方法采用HPLC法，色谱条件为：AglinentC18ODS柱，甲醇-乙腈-水（45：20：35）为流动相，流速1．0ml／min，检测波长294nm，柱温25℃。结果和厚朴酚在0．136-2．720μg（r=0．9998），厚朴酚在0．197~3．940μg（r=0．9992）范围内与峰面积呈良好的线性关系；平均加样回收率分别为98．37％、97．97％，RSD分别为1．81％、2．57％。结论本方法简便、准确、可控，为潜朴药材的质量控制提供了依据。

Content Determination of Honokiol and Magnolol in Magnolia Officinalis by High Performance Liquid Chromatography

Objective To establish a method for content determination of honokiol and magnolol in Magnolia officinalis,simultaneously.Methods High performance liquid chromatography(HPLC) was employed.Chromatographic conditions were as follows: Aglinent C18 ODS column(4.6 mm×250 mm,5 μm) was used,MeOH-Acetonitrile-water(45∶20∶35) was used as mobile phase,the flow rate was 1.0 ml/min,detection wavelength was set at 294 nm,column temperature was at 25℃.Results There was a good linear relationship between the peak area and sampling size of honokiol within the range of 0.136 to 2.72 μg(r=0.999 8),and magnolol within the range of 0.197 to 3.94 μg(r=0.999 2),respectively.The average recoveries of honokiol and magnolol were 98.37%(RSD=1.81%)and 97.97%(RSD=2.57%),respectively.Conclusion This method can provide a basis for the quality control of Magnolia officinalis in Qianshan county,Anhui province,because of its being simple,accurate and controllable.