KLF14基因rs972283多态性与2型糖尿病的关系研究

目的 研究KLF14基因rs972283多态性与宁夏2型糖尿病的关系.方法 用聚合酶链反应- 限制性片段长度多态性的方法（RFLP- PCR）,对宁夏2型糖尿病患者（DM组）和正常对照组（NC组）KLF14基因rs972283位点进行基因多态性和等位基因频率分析,测定相应生化指标.结果 KLF14基因rs972283位点多态性在DM组和NC组的分布差异无统计学意义（P＞0.05）;DM组内携G等位基因患者的体质指数（BMI）、腰臀比（WHR）、血胆固醇（TC）、胰岛素抵抗指数（HOMA-IR）均高于携A等位基因者,DM组内携G等位基因者胰岛β细胞功能（HOME-β）较携A等位基因者低,差异均有统计学意义（P＜0.05）.结论 KLF14基因rs972283位点多态性可能与宁夏2型糖尿病发病无关,与肥胖、血脂代谢等有关.

Relationship between polymorphism of KLF14 rs972283 and type 2 diabetes in Ningxia

Objective To investigate the relationship between polymorphism of KLF14 rs972283 and type 2 diabetes mellitus （T2DM） in Ningxia. Methods Polymerase chain reaction - restriction fragment length polymorphism （ PCR - RFLP） and case - control was used to analyze the KLF14 rs972283 and measure the clinical parameters in T2DM and the controls. Results There were no significant differences in genotypic and allelic frequ- encies of the KLF14 rs972283 polymorphism in T2DM and controls. The BMI, WHR, TC and HOMA - IR in patients with G allele of T2DM were significantly higher than those in the controls. The HOMA - 13in patients with G allele of T2DM were significantly lower than those in the control （ P 〈 0. 05 ）. Conclusion The polymorphism of KLF14 rs972283 may have no relation with T2DM in Ningxia, but it may have relation with obesity and lipids metabolism.