枸杞多糖对人白血病K562细胞株凋亡作用的基础研究

目的 探讨枸杞多糖（LBP-X）抑制人慢性粒细胞白血病（CML）K562细胞增殖的作用机制,研究LBP-X对K562细胞凋亡的影响,探讨LBP-X与三氧化二砷（As2O3）诱导K562细胞凋亡的剂量对应关系.方法 用LBP-X、As2O3处理K562细胞,四氮甲唑蓝（MTT）比色法检测药物对细胞的抑制作用,倒置显微镜观察细胞形态变化,流式细胞仪分析细胞DNA含量变化及凋亡细胞的比例.结果 LBP-X对K562细胞的IC50约为227.50 μg/ml,经LBP-X作用后,K562细胞呈现凋亡的形态学改变.流式细胞仪可在2倍体前检测到凋亡峰,凋亡细胞比例为8.43%-57.92%.结论 LBP-X对K562细胞有抑制作用,并能诱导其发生凋亡.LBP-X对K562细胞的效能和效价强度约为As2O3的1/1 000.

Effect of Lycium Barbarm Polysaccharide on apoptosis of human leukemia K562 cell

Objective To study the effects of Lycium Barbarum Polysaccharide （ LBP - X） on apoptosis of human leukemia K562 cell line in vitro. To explore the dosage relationship between LBP - X and As203. Methods Chronic Myeloginous leukemia （CML） K562 cell line was treated with LBP - X on different concentration and acting - time. The inhibition rates of cell reproduction and IC50 （inhibiting concentration of 50% ） were detected with MTY method. The changes of morphology were observed with inverted microscopy. Flow hyetometer（FCM） was used to detect DNA quantity of the cells and the percentage of cell apoptosis. Results The IC50 of LBP - X for K562 cells was 227.50 p~g/ml. Under the influence of LBP - X , K562 cells exhibited morphological changes of ap- optosis. Flow cytometer assay： K562 cells exposed to LBP -X showed apoptotic peaks. The ratio of apoptosis was from 8% to 60% in the experiment rage. Conclusion Our study show that human Chronical Myeloginous leukemia （CML） K562 cells can be apoptoted in vitro by LBP - X. The effect of As203 on K562 is 1000 times compared with LBP - X.