脆性部位与原癌基因在乙型肝炎病人中的相关性

①目的 探讨脆性部位与原癌基因在乙型病毒性肝炎（乙肝）病人中的相关性及其与原发性肝癌发生的关系。②方法 用低叶酸、低小牛血清和较高ｐＨ值培养方法，常规外周血染色体制片，对２０例乙肝病人（病人组）和２０例健康正常人（对照组）进行染色体脆性部位分析。③结果 病人组检出３１种脆性部位，主要分布于Ａ，Ｂ，Ｃ组染色体，其表达率为８．９４％，与正常对照组（４．０５％）比较差异有显著性（ｕ＝５．８３，Ｐ〈０．０

A STUDY OF RELATIONSHIP BETWEEN FRAGILE SITE AND PROTO-ONCOGENE IN THE HEPATITIS B PATIENTS

Objective\ To study the relationship between the fragile site and proto oncogene in the hepatitis B patients and its influence on the emergence mechanism of primary hepatocellular carcinoma(PHC).\ Methods\ A peripheral blood preparation of chromosome was made routinely. The culture of cells was done in low concentration of serum, folate and higher pH medium. 20 patients with hepatitis B as a group and healthy persons as control group. The fragile sites of the 2 groups were exactly located and analyzed.\ Results\ The fragile sites of the hepatitis B patients group were determined as 31 species mainly distributed on the chromosomes of A, B, C group. The expression rate of the fragile site (8.94%) was higher than that (4.05%) of the control. There was a striking difference between them ( u=5.83,P <0.001 ). Among the fragile sites there were 12 which overlaped the proto oncogene sites and the superposition frequency was 38.7%. Fra 2q21 overlapped two proto oucogene sites, fra 11q13 overlapped five proto oncogene site.\ Conclusion\ In hepatitis B patients, the overlap of the fragile site with the proto oncogene site can easily lead to HBV DNA integrate and play an important role in inducing PHC by activating proto oncogene.