贫血对肿瘤化疗疗效影响的实验研究

目的：在小鼠实体瘤模型中研究贫血对化疗疗效的影响。方法：用单次剂量的卡铂(50mg／kg)静推造成Hb较长时间的降低。在另一组中，用卡铂前7天始予以rHuEPO(1000IU/kg)皮下注射3次/周预防贫血的发展。两组均于卡铂用后的第4天自小鼠的右侧腋部皮下植入肿瘤(S180—小鼠肉瘤)。植入肿瘤后的第5天用单次剂量的CTX(60mg／kg)静脉注射。结果：rHuEPO可以防治化疗所致的贫血。化疗后可见到肿瘤生长受到抑制，随后肿瘤又再生长。贫血组的肿瘤生长延缓时间要短于非贫血组(6．3天比9．3天)，被纠正的贫血组的肿瘤生长延迟时间与对照组大致相仿(9．3天)。结论：贫血可导致肿瘤化疗疗效的降低，其机制可能与肿瘤组织的低氧供有关。而通过rHuEPO纠正贫血可望提高化疗的疗效。

Experimental Study on the Impact of Anemia upon the Efficacy of Chemotherapy in Mice Tumors

Objective:To examine the impact of anemia on the efficacy of chemotherapy in solid experimental tumors.Methods:Anemia was induced using a single dose of carboplatin (50mg/kg,iv) resulting in a long lasting reduction of the hemoglobin concentration. In a second group, the development of anemia was prevented by rHuEPO( 1 000 U/kg) administered s.c three times/week starting 7 days before carboplatin application. Four days after carboplatin treatment, tumors (S 180 sarcoma of the mice) were implanted s.c under the right axilla. Tumors were treated with a dose of cyclophosphamide (60 mg/kg,iv) 5 days after implantation.Results:Chemotherapy induced anemia can be prevented by rHuEPO. A growth delay with a subsequent regrowth of the tumors was observed after chemotherapy. The growth delay in anemia group was significantly shorter compared with non anemic controls (6 3 days versus 9 3 days). In the group where anemia was prevented by rHuEPO treatment, growth delay was comparable with that of non anemia controls (9 3 days).Conclusion:Anemia reduces efficacy of chemotherapy in tumors, probably as a result of a poorer oxygen supply to tumor tissues, whereas correction of anemia by r HuEPO treatment may increase the efficacy of chemotherapy.