白细胞介素1家族在翼状胬肉上皮内的异常表达

目的：了解白细胞介素1基因家族[白细胞介素(interleukin,IL)1α、IL-1β、IL-1受体拮抗剂(inter-leukine-1 receβtor antagonist,IL-1RA)]及IL-lβ前体(interleukine-1β precursor)在翼状胬肉上皮内的表达。方法：用免疫荧光组织化学方法对10例初发性翼状胬肉患者切除的翼状胬肉组织中IL-1α、IL-1β、IL-lβ前体及IL-lBA蛋白进行检测，并与正常人结膜组织进行对照。结果：免疫荧光组织化学染色显示，在正常结膜上皮中，IL-1α及IL-lβ全层有弱阳性表达，上皮细胞表达较基底细胞明显，IL-lβ前体只在上皮细胞表层表达，IL-lBA则无明显的表达。10例翼状胬肉患者中7例上皮的IL-1α、IL-lβ、IL-lβ前体及IL-lRA表达与正常结膜明显不同，表现为IL-lα、IL-lβ、IL-lβ前体较正常明显增加，IL-lα及IL-lβ在全层呈强阳性，且基底细胞与上皮细胞的表达强度基本一致，IL-lRA表层细胞呈阳性表达，3例IL-lα、IL-lβ、IL-lβ前体及IL-lBA表达较正常结膜稍强。结论：翼状胬肉组织上皮IL-lα、IL-lβ、IL-lβ前体及IL-lBA表达增加，提示翼状胬肉可能处于轻微的非感染炎症状态。眼科学报2003；19：133-136

The Abnormal Expression of Interleukine-1 Family in Pterygium

PURPOSE: To evaluate the expression of interleukine 1 family including interleukin-1, (IL-1 alpha), interleukin-1 beta (IL-1 beta), interleukine-1 receptor antagonist, (IL-1RA) and interleukine-1 beta precursor by the epithelium in pterygium so as to understand the pathogenesis of this disorder. METHODS: The immunoflurescent staining was performed in 10 patients with primary pterygium, compared to 10 normal conjunctival epithelium, to determine the expression of IL-1 alpha, IL-1 beta, IL-1 beta RA and IL-1 beta precursor proteins RESULTS: The IL-1 alpha and IL-1 beta were present at whole epithelium while the IL-1 beta precursor was expressed by the superficial cells in normal conjunctival epithelium in immunofluorescent staining. No IL-1RA positive staining was found in normal conjunctiva. Of 10 pterygia, 7 pterygias were stained by IL-1 alpha and IL-1 beta in the full thickness of epithelium, IL-1 beta precursor and IL-1RA in the superior cell and with strong staining compared to control group, 3 of them showed the similar staining pattern with normal conjunctiva. CONCLUSION: The increasing IL-1 alpha, IL-1 beta, IL-1 beta RA and IL-1 beta precursor proteins are presented in pterygium, which shows that the pterygium is in the state of no-infective inflammation.