| Expression of Bcl-2 induced byIGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors |
| |
| 引用本文： | ZHU Xiao-feng 1,XU Zhong-ye 1,ZHANG Xiao-mei 1,ZHOU Hou-lun 2 (1.Second Affiliated Hospital,Jiamusi University,Jiamusi 154002,China,2.Tongji Medical College,Huazhong University of Science and Technique,Wuhan 430030,China.). Expression of Bcl-2 induced byIGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors[J]. 黑龙江医药科学, 2003, 26(5): 7-9 |
| |
| 作者姓名： | ZHU Xiao-feng 1 XU Zhong-ye 1 ZHANG Xiao-mei 1 ZHOU Hou-lun 2 (1.Second Affiliated Hospital Jiamusi University Jiamusi 154002 China 2.Tongji Medical College Huazhong University of Science and Technique Wuhan 430030 China.) |
| |
| 作者单位： | 1.Second Affiliated Hospital，Jiamusi University，Jiamusi 154002，China;2.Tongji Medical College，Huazhong University of Science and Technique，Wuhan 430030，China. |
| |
| 基金项目： | Supported by National Natural Science Fundation of China( 30 1 70 963) |
| |
| 摘 要： | 0 introductionNeural stem cells (NSCs) replacem ent therapies for cen-tral nervoussystem injuriesand neurodegenerative disease holdfine promising. However,m any injury factors exist in trans-plant region,so enhancing the survival ability of grafted NSCsis very important.Bcl- 2 is unique am ong proto- oncogenesin that itfunctions as a repressor of programm ed cell death[1 ] .In the developing nervons system,Bcl- 2 protein arewidespreadly expressed in proliferating stem cells,it can pro-tec…
|
| 关 键 词： | 神经干细胞 中枢神经损伤 细胞因子 诱导 IGF-1 Bcl-2 |
Expression of Bcl-2 induced by IGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors |
| |
| ZHU Xiao-feng,XU Zhong-ye,ZHANG Xiao-mei,ZHOU Hou-lun. Expression of Bcl-2 induced by IGF-1 in NSCs and protective effect of Bcl-2 on NSCs deprived of browth factors[J]. Heilongjiang Medicine and Pharmacy, 2003, 26(5): 7-9 |
| |
| Authors: | ZHU Xiao-feng XU Zhong-ye ZHANG Xiao-mei ZHOU Hou-lun |
| |
| Abstract: | Objective: To study the effect of IGF-1 on the expression of Bcl-2 of NSCs and the protective effect of Bcl-2 on the NSCs deprived of browth factors. Methods: The neural stem cells wereisolated from hippocampi of newborn rats and were cultured by using serum-free medium containing EGF 20ng/ml and bFGF 20ng/ml. Expression of Bcl-2 and nestin antigen were observed by using immunocytochemistry method, MTT method was used to analyse the vitality change of NSCs cultured with IGF-1 and without IGF-1 after withdrawing growth factors. Results: After cultured 7d with IGF-1,the cells were almost positive expression of nestin antigen, and (21.3±0.4)% of all NSCs were Bcl-2 positive cells, furthermore, these cells had a larger survival term against withdrawing growth factors than that cultured without IGF-1.Conclusion: IGF-1 could promote the expression of Bcl-2 in NSCs without affecting the characters of positive expression of nestin antigen, moreover, could increase the self-protection ability through elevating expression of Bcl-2 against deprivation of growth factors in the NSCs. |
| |
| Keywords: | neural stem cells IGF-1 Bcl-2 protection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
