藤三七总皂苷的大孔树脂纯化工艺优选

目的： 优选藤三七总皂苷的大孔树脂分离纯化工艺。 方法： 采用UV测定总皂苷含量。通过静态吸附-洗脱试验筛选大孔树脂型号；以总皂苷动态吸附量和洗脱率为指标，利用正交试验优选藤三七总皂苷的大孔吸附树脂纯化工艺条件。 结果： 选用D101型大孔吸附树脂分离纯化藤三七总皂苷，最佳工艺条件为上样液质量浓度0.1 g·mL^-1，上样流速1 BV·h^-1，径高比1：10，上样量（药材量-树脂）0.2 g·mL^-1，乙醇体积分数40%，乙醇用量8 BV，洗脱流速2 BV·h^-1；藤三七总皂苷纯度>50%。 结论： 优选的纯化工艺合理可行，可较好地分离纯化藤三七总皂苷。

Optimization of Purification Technology for Total Saponins from Anredera cordifolia by Macroporous Resin

Objective: To optimize separation and purification process of total saponins from Anredera cordifolia by macroporous resin. Method: The content of total saponins was determined by UV.Model of macroporous resin was screened by static adsorption-elution test;Orthogonal test was adopted to optimize separation and purification process of total saponins from A. cordifolia by taking dynamic adsorption capacity and elution rate as indexes. Result: D101 macroporous resin was selected,its optimum separation and purification process conditions were determined as follows:the concentration of sample solution 0.1 g·mL^-1,adsorption velocity 1 BV·h^-1,ratio of diameter-high of cation resin column 1:10,quality proportion of crude drug-resin 0.2 g·mL^-1,ethnol concentration 40%,elution velocity 2 BV·h^-1,eluent volume 8 BV;Purity of total saponins from A. cordifolia under these conditions could reach above 50%. Conclusion: Optimized separation and purification process was reasonable and feasible,it could easily separate and purify total saponins from A. cordifolia.