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Cryopreserved Porcine Hepatocytes: Expression and Induction of Cytochrome P450, Isoform CYP2E1 |
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| Authors: | E Baldini R Cursio G DeSousa J Honiger P Bayer R Rahmani J Gugenheim |
| Institution: | a Laboratoire de Recherches Chirurgicales, IFR 50, Faculté de Médecine, Université de Nice Sophia Antipolis, Nice, France b Service de Chirurgie Digestive et Transplantation Hépatique, Hôpital l'Archet 2, Université de Nice Sophia Antipolis, Nice, France c Service d'Anatomo-Pathologie, Hôpital Pasteur, Université de Nice Sophia Antipolis, Nice, France d Laboratoire de Pharma-Toxicologie Cellulaire et Moléculaire, INRA, Antibes, France e INSERM U 420, Université de Paris V, Paris, France |
| Abstract: | Cryopreservation of porcine hepatocytes for their use in bioartificial liver devices may result in reduced cytochrome P450 (CYP) enzyme activity. The aim of this study was to assess the effects of several CYP inducers on the isoform CYP2E1 protein expression in cryopreserved porcine hepatocytes. Isolated porcine hepatocytes were cryopreserved for 1 month, thawed, and cultured for 3 days. During medium culture, the hepatocytes were exposed to the following CYP inducers: dimethyl sulfoxide, rifampicin, phenobarbital, 3-methylcholanthrene, and dexamethasone. CYP2E1 protein expression was determined by immunoblotting. CYP2E1 protein levels were constantly detected in cryopreserved porcine hepatocytes. CYP inducers did not modify CYP2E1 protein levels. Long-term cryopreserved porcine hepatocytes preserved their capacity for CYP2E1 protein expression, although exposure of these hepatocytes to CYP inducers did not modify the CYP2E1 protein expression. |
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