实验性糖尿病结核动物模型的建立

目的　建立糖尿病合并结核的动物模型。方法 腹腔一次性注射2%链脲佐菌素(60mg/kg体重)建立Wistar大鼠糖尿病模型,再经大鼠尾静脉注射结核分支杆菌H₃₇Rv0.01mg/0.4ml建立糖尿病结核模型。分别测定血糖值、肺脏病变指数和肺泡巨噬细胞吞噬的结核分支杆菌数,观察肺、肝、脾及胰腺组织的病理学改变。结果 实验组不仅血糖≥16.65mmol/L,胰岛受损,且肺、脾组织有明显结核病变。结论 实验表明我们建立的糖尿病结核动物模型是成功的。

Animal model of experimental diabetes complicated with tuberculosis

Objective To establish an animal model of diabetes complicated with tuberculosis.Methods Mycobacterium tuberculosis H 37 Rv (0.01mg/0.4ml) was injected into Wistar rat with diabetes which was produced by injection of STZ (60mg/kg).Not only blood glucose,index of pulmonary tubercular lesion and count of Mycobacterium tuberculosis in alveolar macrophages were measured,but histopathological changes in lung,liver,spleen and pancreas tissues were also observed.Results In the control group,blood glucose was increased more than 16.65mmol/L,pancreas tissues were injured and typical tuberculosis lesions were found in the lung and spleen.Conclusion It suggested that the animal model of diabetes complicated with tuberculosis was successfully established.