| 丹参酮ⅡA在大鼠肺脏和肝脏细胞器的分布 |
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| 引用本文： | 王颖,吴昭怡,覃小玲,陈孝. 丹参酮ⅡA在大鼠肺脏和肝脏细胞器的分布[J]. 现代食品与药品杂志, 2011, 0(11) |
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| 作者姓名： | 王颖 吴昭怡 覃小玲 陈孝 |
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| 作者单位： | 中山大学附属第一医院药学部; |
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| 基金项目： | 广东省自然科学基金(编号:8151008901000167); 广东省中医药局建设中医药强省科研课题重点项目(编号:2009159); 广东省医院药学研究基金(编号:2011A25) |
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| 摘 要： | 目的考察丹参酮ⅡA在大鼠肺脏、肝脏细胞器的分布,以探讨丹参酮ⅡA在大鼠肺脏高浓度储留的机制。方法雄性SD大鼠经颈静脉给予6 mg/kg丹参酮ⅡA,给药后分批处死,取其肝、肺组织,差速离心法分离细胞器,高效液相色谱-串联质谱(LC-MS/MS)法测定丹参酮ⅡA的浓度。结果给药后,丹参酮ⅡA在肺脏的浓度远高于在肝脏的浓度。丹参酮ⅡA在肝脏细胞器中主要以结合型的形式存在,在肺脏中则是原型稍多。丹参酮ⅡA在大鼠肝脏的微粒体和溶酶体部分分布较多,但是无明显的蓄积部位;而在肺脏丹参酮ⅡA主要分布于细胞器的轻线粒体部分。结论丹参酮ⅡA主要蓄积于肺脏细胞器的轻线粒体部分,而在肝脏细胞器无明显蓄积部位。这是TSⅡA在大鼠肺脏细胞器蓄积的可能机制。
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| 关 键 词： | 丹参酮ⅡA 药代动力学 细胞器分布 |
Subcellular Distribution of Tanshinone IIA in Rat Lung and Liver |
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| WANG Ying,WU Zhao-yi,QIN Xiao-ling,CHEN Xiao. Subcellular Distribution of Tanshinone IIA in Rat Lung and Liver[J]. JOurnal of Modern Food and Pharmaceuticals, 2011, 0(11) |
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| Authors: | WANG Ying WU Zhao-yi QIN Xiao-ling CHEN Xiao |
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| Affiliation: | WANG Ying,WU Zhao-yi,QIN Xiao-ling,CHEN Xiao(Department of Pharmacy,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510080,China) |
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| Abstract: | Objective To study the subcellular distribution of tanshinone IIA in rat lung and liver.Methods After intravenous administration of 6 mg/kg tanshinone IIA,the rats were sacrificed after dosing.Cell fractions of the lung and liver tissue were produced by differential centrifugation.Tanshinone IIA concentrations were determined using the validated LC-MS/MS method.Results Tanshinone IIA concentrations in lung and its organelles were much higher than that in liver and its organelles after administration.Tanshin... |
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| Keywords: | tanshinone IIA pharmacokinetics subcellular distribution |
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