| 丙酸氟替卡松对鼻黏膜上皮细胞表达的天然免疫分子的免疫调节作用 |
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| 引用本文： | 黄思海,郑静,郭洁波,陈枫虹,陈德华,李华斌. 丙酸氟替卡松对鼻黏膜上皮细胞表达的天然免疫分子的免疫调节作用[J]. 现代食品与药品杂志, 2011, 0(10) |
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| 作者姓名： | 黄思海 郑静 郭洁波 陈枫虹 陈德华 李华斌 |
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| 作者单位： | 江苏省阜宁县人民医院耳鼻喉科;中山大学附属第一医院耳鼻咽喉科医院; |
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| 基金项目： | 国家自然科学基金(编号:30872845,81070771) |
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| 摘 要： | 目的检测丙酸氟替卡松对人鼻黏膜上皮细胞表达天然免疫分子的影响。方法 采用丙酸氟替卡松和/或聚肌胞苷酸(Polyinosinic:polycytidylic acid,Poly I:C)、细菌脂多糖(Lipopolysaccharides,LPS)等刺激离体培养的鼻黏膜上皮细胞后,通过实时定量PCR检测Toll样受体(Toll like receptor,TLR)的表达,采用酶联免疫吸附试验检测细胞培养上清中血清淀粉样蛋白A(Serum amyloidA,SAA)、骨桥蛋白(Osteopontin,OPN)等的表达水平。结果丙酸氟替卡松单独刺激能够增加鼻黏膜上皮细胞表达TLR-3 mRNA(7.5倍)和TLR-4 mRNA(4.5倍),Poly I:C单独刺激能够增加鼻黏膜上皮细胞表达TLR-3 mRNA 28.5倍,LPS单独刺激能够增加鼻黏膜上皮细胞表达TLR-4 mRNA 18.4倍,丙酸氟替卡松刺激后细胞培养上清中SAA和OPN水平也出现显著增加。丙酸氟替卡松和Poly I:C以及LPS对增加细胞培养上清中SAA和OPN水平具有协调效应。结论丙酸氟替卡松能够增强鼻黏膜上皮细胞的天然免疫反应。
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| 关 键 词： | 丙酸氟替卡松 Toll样受体 血清淀粉样蛋白A 骨桥蛋白 上皮细胞 |
Effect of Fluticasone Propionate on Molecules for Innate Immunity in Human Nasal Epithelial Cells |
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| HUANG Si-hai,ZHENG Jing,GUO Jie-bo,CHEN Feng-hong,CHEN De-hua,LI Hua-bin. Effect of Fluticasone Propionate on Molecules for Innate Immunity in Human Nasal Epithelial Cells[J]. JOurnal of Modern Food and Pharmaceuticals, 2011, 0(10) |
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| Authors: | HUANG Si-hai ZHENG Jing GUO Jie-bo CHEN Feng-hong CHEN De-hua LI Hua-bin |
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| Affiliation: | HUANG Si-hai1,ZHENG Jing2,GUO Jie-bo2,CHEN Feng-hong2,CHEN De-hua2,LI Hua-bin2(1.Department of Otolaryngology of Funing People's Hospital,Funing,Jiangsu 224400,China,2.Otorhinolaryngology Hospital,the First Affliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong 510080,China) |
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| Abstract: | Objective To investigate the effects of fluticasone propionate on molecules for innate immunity in human nasal epithelial cells.Methods The vitro culture nasal epithelial cells were stimulated by fluticasone propionate and Polyinosinic:polycytidylic acid(Poly I:C).Toll like receptor(TLR) were examined by real-time PCR,and serum amyloid A(SAA) and osteopontin(OPN) in supernatant were determined by enzyme-linked immunosorbent assay.Results The TLR-3 mRNA expression(7.5 times) and TLR-4 mRNA expression(4.5 tim... |
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| Keywords: | fluticasone propionate toll like receptor serum amyloid A osteopontin epithelial cells |
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