复圣散对血管内皮细胞活性及其与中性粒细胞粘附率的影响

探讨中性粒细胞与血管内皮细胞的粘附特性的影响因素，以及中药复圣散的干预作用。方法；以培养人脐静脉内皮细胞作为靶细胞，观察在高脂，高糖，肿瘤坏死因子及缺氧再氧化条件下，对培养的ＶＥＣ活性以及ＰＭＮ与ＶＥＣ相互粘附的影响。结果：高脂，高糖，ＴＮＦ－α和缺氧再氧化等均可抑制ＶＥＣ活性，并显著增强ＰＭＮ与ＶＥＣ的相互粘附作用，其粘附率正常对照组为１００％，高脂组则为１６７．７％，缺氧再氧化组为１５１．４％

Effect of Fusheng Powder on Activity of Vascular Endothelial Cells and Its Adhesion to Polymorphonuclear Neutrophils

OBJECTIVE: To explore the effect of Fusheng powder on the adhesion of polymorphonuclear neutrophils (PMN) in human peripheral blood to vascular endothelial cells (VEC). METHODS: Using cultured human umbilical vein cells as target cells, while being incubated with high glucose (HG, 30 mmol/L), high-lipid (HL, 20%), tumor necrosis factor-alpha (TNF-alpha, 10%) and hypoxia (95% N2, 5% CO2, 37 degrees C, 30 min, and then in the air 30 min) for 24 h, respectively, the activity of VEC and the adhesive effect of PMN to VEC were surveyed. RESULTS: It revealed that HG, HL, TNF-alpha and hypoxia could enhance obviously the adhesion of PMN to VEC, the cell attachment rate in normal control, HG group, TNF-alpha, hypoxia and HL after 30 minutes contact were 100%, 129.6%, 136.7%, 151.4% and 167.7% (all P < 0.01, compared with control group) respectively, but high and low dosage of the Fusheng powder could significantly inhibit the adhesion of PMN to VEC. CONCLUSIONS: HG, HL, TNF-alpha and hypoxia could all activate EC respectively and enhance the adhesion of PMN to VEC, but the Fusheng powder could protect VEC and partly block the effect of this adhesion. However, the effects of Fusheng powder on anti-adhesion are of great importance in clinical cardiovascular and cerebral vascular diseases.