| HLA-DRB PCR-单链构象多态性分析 |
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| 引用本文： | 兰炯采,钱新华.HLA-DRB PCR-单链构象多态性分析[J].中国输血杂志,1994,7(2):61-63. |
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| 作者姓名： | 兰炯采 钱新华 |
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| 作者单位： | 中国医学科学院输血研究所，中国医学科学院输血研究所，中国医学科学院输血研究所，第一军医大学南方医院，第一军医大学南方医院 610081，610081，610081 |
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| 摘 要： | 采用聚合酶链技术(PCR)扩增10例HLA-DR血清学定型者的DRB基因,然后作单链构象多态性(SSCP)分析,发现不同基因型的DNA单链区带数及电泳速度不同,提示本方法可用于器官移植时供、受者DRB基因配型及法医犯罪识别。
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| 关 键 词： | HLA—DR PCR(聚合酶链反应) SSCP(单链构象多态性) |
DRB PCR-Single-Strand Coaformation Polymorphism Analysis of HLA-DR |
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| Lan Jioncai,et al.DRB PCR-Single-Strand Coaformation Polymorphism Analysis of HLA-DR[J].Chinese Journal of Blood Transfusion,1994,7(2):61-63. |
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| Authors: | Lan Jioncai |
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| Abstract: | DRB alleles of 10 different HLA-DR individuals tested by serology typing were studied with polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, single-strand DNA of different sequence exhibited specific band patterns and mobilities by this technique. Our results suggested that PCR-SSCP is expected to be a useful tool for donor-recipient DRB allele HLA-matching in organ transplantation, as well as identification of criminal in forensic medicine. |
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| Keywords: | HLA-DR PCR (Polymerase Chain Reaction) SSCP (Single-Strand Conformation Polymorphism) |
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