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The presence of IgE on macrophages and dendritic cells infiltrating into the skin lesion of atopic dermatitis
Authors:D Y Leung  E E Schneeberger  R P Siraganian  R S Geha  A K Bhan
Institution:1. Division of Allergy, Children''s Hospital Medical Center, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115 USA;2. Division of Immunology, Children''s Hospital Medical Center, Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115 USA;3. Department of Pathology, Massachusetts General Hospital, Boston, Massachusetts 02114 USA;4. Harvard Medical School, Boston, Massachusetts 02114 USA;5. National Institutes of Health, Bethesda, Maryland 20205 USA;1. Department of Burns, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China;2. School of Biomedical Engineering, Research and Engineering Center of Biomedical Materials, Anhui Provincial Institute of Translational Medicine, Anhui Medical University, Hefei 230032, China;3. Division of Gastroenterology, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, China;4. The Second Clinical Medical College, Anhui Medical University, Hefei 230022, China;5. Department of Oncology, the First Affiliated Hospital of Anhui Medical University, Hefei 230022, China;6. College and Hospital of Stomatology, Anhui Medical University, Key Lab. of Oral Diseases Research of Anhui Province, Hefei 230032, China;1. Center for the Study of Itch and Sensory Disorders, Washington University School of Medicine, St Louis, Mo;2. Division of Dermatology, Department of Medicine, Washington University School of Medicine, St Louis, Mo;3. Division of Allergy and Immunology, Department of Medicine, Washington University School of Medicine, St Louis, Mo;4. Division of Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine, St Louis, Mo;5. Kimberly and Eric J. Waldman Department of Dermatology, Icahn School of Medicine at Mount Sinai, New York, NY;6. Mark Lebwohl Center for Neuroinflammation & Sensation, Icahn School of Medicine at Mount Sinai, New York, NY;7. Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY;8. Tisch Cancer Institute, Icahn School of Medicine at Mount Sinai, New York, NY;9. Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY;10. Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY;11. The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Md;12. Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, Md;13. LEO Pharma A/S, Ballerup, Denmark;14. Department of Dermatology, The First Affiliated Hospital, Sun Yat-sen University, Guangdong, China;15. Allen Discovery Center for Neuroimmune Interactions, New York, NY;1. Cardiovascular Research Laboratory, Division of Cardiology, Pneumology and Angiology, Medical Faculty, Heinrich Heine University of Düsseldorf, Düsseldorf, Germany;2. Institute for Pharmacology and Toxicology, Ruhr-University Bochum, Bochum, Germany;1. Department of Rehabilitation Medicine and Laboratory of Liver Surgery, Sichuan University-University of Oxford Huaxi Joint Centre for Gastrointestinal Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China;2. Key Laboratory of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China;3. West China School of Pharmacy, Sichuan University, Chengdu, China;4. Department of Plastic Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China;5. Department of Gynecology and Obstetrics, Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital, Chengdu, China
Abstract:Monoclonal antibodies reactive with human IgE were used to investigate the presence of surface IgE in situ on mononuclear cells infiltrating into the skin lesion of atopic dermatitis (A.D.) by application of the immunoperoxidase technique to tissue sections for light and electron microscopic examination. A substantial proportion of infiltrating macrophages but not of lymphocytes were found to bear IgE on their cell surfaces. These observations raise the possibility that IgE may contribute to the skin inflammation associated with A.D. via non-mast-cell-mediated immune mechanisms. We hypothesize that allergens introduced into the skin lesion of A.D. are potentially capable of interacting not only with IgE-bearing mast cells but also with IgE-bearing macrophages and dendritic cells to cause the release of inflammatory mediators.
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