高尿酸血症大鼠肾脏抗氧化酶活力的实验研究

目的 建成可靠的高尿酸血症（HUA）大鼠模型，研究HUA大鼠血清中氧化应激相关指标及肾功能的变化。方法 采用单一酵母喂饲和氧嗪酸联合酵母喂饲两种方法建成可靠的HUA大鼠模型，检测尿酸（UA）、尿素氮（BUN）、肌酐（CREA）、过氧化氢的含量及超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）的活力水平。结果 酵母组和氧嗪酸酵母组UA、BUN、CREA值均高于对照组（P<0.05）；酵母组和氧嗪酸酵母组SOD活力明显低于对照组（P<0.05）；相比对照和酵母组，氧嗪酸酵母组GSH-Px活力明显降低（P<0.05）；过氧化氢差异无统计学意义（P>0.05）。结论 单一酵母喂饲和氧嗪酸联合酵母喂饲均可建成可靠的HUA大鼠模型；两种喂饲方法诱导的HUA模型大鼠机体处于氧化应激状态，肾功能出现损害。

Experimental Study on Antioxidant Enzyme Activity in Kidney of Rats with Hyperuricemia

Objective HUA rat model was established to study the changes of serum oxidative stress and renal function in HUA rats. Methods A reliable HUA rat model was established by single yeast feeding and oxoxalic acid combined with yeast feeding.Detection of the vitality level of UA,CREA,BUN,SOD,GSH-Px and hydrogen peroxide was done. Results The UA, BUN and CREA values of yeast group and oxymatrine group were higher than those of control group (P<0.05).The activity of SOD in yeast group and oxazinium yeast group was significantly lower than that in control group (P<0.05).Compared with yeast and control, GSH-Px activity was significantly decreased (P<0.05), but there were no differences in hydrogen peroxide (P>0.05). Conclusion A reliable HUA rat model can be constructed by two methods:single yeast feeding and aerobic ammonium sulfate combined with yeast feeding.Two kinds of feeding methods induced HUA model rats were in oxidative stress state, renal function damage.