红景天甙联合碳酸钙对老年雌性大鼠骨量、骨代谢和氧化应激水平影响

目的探索红景天甙联合碳酸钙对老年雌性大鼠骨量、骨代谢和氧化应激水平影响。方法将40只24月龄雌性Sprague-Dawley(SD)大鼠随机分为模型组(OP组),红景天甙组(H组),碳酸钙组(Ca组)和红景天甙联合碳酸钙组(H+Ca组),而将10只3个月大鼠作为对照组(NC组)。观察各组大鼠腰椎和双侧股骨骨密度和骨矿物质水平的变化。测定各组大鼠骨代谢指标、超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)水平变化。结果与OP组比较,H、Ca和H+Ca组大鼠腰椎骨密度,双侧股骨骨密度和骨矿物质水平显著升高;治疗12周,H、Ca和H+Ca组血清I型胶原C端肽(CTX-1)、骨钙素、碱性磷酸酶(ALP)、I型前胶原N端肽(PINP)和抗酒石酸酸性磷酸酶5b(TRACP-5b)水平均显著降低,比较差异有统计学意义(P 0.05);治疗12周,H、Ca和H+Ca组血清SOD和GSH-Px活性明显升高,MDA含量显著降低,差异有统计学意义(P 0.05或P0.01);其中H+Ca组上述指标变化最为显著;差异有统计学意义(P 0.05)。结论红景天甙联合碳酸钙可显著提高老年大鼠的抗氧化能力,增加骨密度,降低骨代谢水平,减少骨矿物质流失,从而预防和治疗骨质疏松症。

Effects of salidroside and calcium carbonate on bone mass, bone metabolism and oxidative stress in aged female rats

Objective To explore the effects of salidroside and calcium carbonate on bone mass, bone metabolism and oxidative stress in aged female rats. Methods Forty female Sprague-Dawley (SD) rats aged 24 months were randomly divided into model group (group OP), melatonin group (group M), calcium carbonate group (group Ca) and melatonin combined with calcium carbonate group (group M+Ca), while 10 rats aged 3 months were set as the control group (group NC). The changes of bone density and bone mineral level of lumbar vertebra and bilateral femur in rats of each group were observed. The levels of serum bone metabolism indexes, superoxide dismutase (SOD), malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) in rats of each group were determined. Results Compared with the OP group, the lumbar vertebrae bone density, bilateral femoral bone density, and bone mineral levels in the H, Ca, and H+Ca groups were significantly increased. After 12 weeks of treatment, serum type I collagen C in the H, Ca, and H+Ca groups The levels of telomere (CTX-1), osteocalcin, alkaline phosphatase (ALP), type I procollagen N-terminal peptide (PINP), and tartrate-resistant acid phosphatase 5b (TRACP-5b) were significantly reduced. (P <0.05); after 12 weeks of treatment, the SOD and GSH-Px activities in the H, Ca, and H+Ca groups increased significantly, and the MDA content decreased significantly; the differences were statistically significant (P<0.05 or P<0.01) Among them, the above indexes of H+Ca group had the most significant changes; the differences were statistically significant (P<0.05). Conclusions Salidroside combined with calcium carbonate can significantly improve the antioxidant capacity of aged rats, increase bone density, reduce bone metabolism, and reduce bone mineral loss, thereby preventing and treating osteoporosis.