中波紫外线对人真皮成纤维细胞中长链非编码RNA表达的影响

目的 对中波紫外线(ultraviolet rays B,UVB)照射后人真皮成纤维细胞(human dermal fibroblasts,HDFs)中差异表达的长链非编码RNA(long non-ucoding RNAs,LncRNA)进行筛选和功能分析.方法 将处于对数生长期8～11代的HDFs分为对照组和UVB ...

The Effect of UVB Radiation on Expression of Long non-coding RNAs in Human Dermal Fibroblasts

Objective To extract the differently expressed long non-coding RNAs(LncRNAs)in Human dermal fibroblasts(HDFs)with UVB radiation,and conduct a screening and functional analysis.Methods The HDFs were selected from 8 to 11 generations in the logarithmic growth phase and were divided into control group and UVB-stress induced premature senescence(UVB-SIPS)group.The UVB-SIPS group was irradiated with subtoxic doses of UVB(10 mJ/cm^2,5 times).Then CCK-8 was used to detect proliferation activity of HDFs.The total RNAs of each group of HDFs were extracted using TRIzol and analyzed by RNA chip.Normalized intensity|log2(Ratio)|≥2 was set to find the differently expressed genes in each groups.And the differentially expressed genes were analyzed by GO analysis and pathway enrichment analysis.Results The ability of proliferation of HDFs was significantly lower after exposure to UVB at subtoxic doses than that of the control group(P<0.05).When the set the fold change(FC)≥2,a total number of 4854 differently expressed lncRNA and mRNA was detected in UVB-induced HDFs,of which the number of lncRNA and mRNA was 3224 and 1630 separately.Bioinformatics analysis suggests that differentially expressed lncRNAs involve a variety of biological functions,and some biological functions are related to photoaging.Conclusion Subtoxic dose of UVB irradiation can significantly alter the expression of lncRNAs and mRNAs in HDFs.These alterations are related to various biological phenomena including skin aging.