探讨实时荧光定量PCR仪用于流感病毒检测的效果分析

目的:探讨实时荧光定量P C R仪器对流感病毒检测效果.方法:选择2019年6月～2020年6月本中心采集的流感病毒咽拭子及血液样本各116份,根据检测方法将其分为三个不同的组别,分别用实时荧光定量P C R仪、普通PCR仪、酶标仪进行检测、最终采用病毒分离培养法进行验证.结果:本次实验研究结果显示,使用实时荧光定量P...

To Explore the Effect Analysis of Real-Time Fluorescence Quantitative PCR for Influenza Virus Detection

Objective:To explore the detection effect of real-time fluorescence quantitative PCR on influenza virus.Methods:116 throat swabs and blood samples of influenza virus collected by the center from June 2019 to June 2020 were selected and divided into three different groups according to the detection method.They were detected by real-time fluorescence quantitative PCR,ordinary PCR and enzyme labeling instrument respectively,and finally verified by virus isolation and culture method.Results:The experimental results show that the sensitivity and accuracy of detecting influenza virus by real-time fluorescence quantitative PCR is higher than that of traditional serological detection.Conclusion:Although the use of real-time fluorescence quantitative PCR instrument in influenza virus detection needs higher cost,using this method can save the detection manpower and shorten the monitoring time of personnel.It can be used in the rapid screening of influenza virus for employees in public places according to the characteristics of PCR detection,thus helping more people to detect pathogens in time and finally launching targeted treatment programs.