9-顺式维甲酸抑制佛波酯诱导人单核细胞系THP-1向巨噬细胞分化

目的探讨视黄醇类X受体特异性激动剂9-顺式维甲酸对佛波酯诱导人单核细胞系THP-1向巨噬细胞分化的影响。方法体外培养人单核细胞系THP-1,经佛波酯诱导分化为巨噬细胞,同时给予9-顺式维甲酸对佛波酯诱导分化进行干预,通过相差显微镜观察细胞形态,MTT比色法检测细胞贴壁率,流式细胞仪检测与单核/巨噬细胞分化有关的细胞表面标志物CD11b和CD36的表达。结果9-顺式维甲酸干预后梭形、分支状细胞明显减少,与佛波酯单独作用组相比细胞贴壁率减少50%(P<0.01),细胞表面标志物CD11b和CD36分别减少50%和28%(P<0.01)。结论视黄醇类X受体特异性激动剂9-顺式维甲酸可明显抑制佛波酯诱导人单核细胞系THP-1向巨噬细胞分化。

9-cis Retinoid Acid Inhibits the Differentiation of THP-1 Human Monocyte Cell Line into Macrophage Induced by Phorbol-12-Myristate-13-Acetate

Aim To investigate the effect of 9-cis-retinoid acid (9-cisRA), a specific agonist of retinoid X receptors (RXR) on the differentiation of THP-1 human monocyte cell induced by phorbol-12-myristate-13-acetate (PMA). Methods Human monocyte cell line THP-1 was cultured and induced to macrophage by PMA treatment. The effect of 9-cisRA on the differentiation induced by PMA was studied. Cell morphology was observed by phase contrast microscope and the adhesion rate of THP-1 was detected by methyl thiazolyltetrazolium (MTT) assay. The cell surface markers involved in differentiation of monocyte/macrophage (CD11b, CD36) were analyzed by flow cytometry (FACS). Results The metamorphotic cells were decreased in 9-cisRA group. THP-1 cell adhesion rate was inhibited by 50% (P<0.01), and the cell surface markers (CD11b, CD36) were decreased by 50% and 28% (P<0.01) in 9-cisRA treated group. Conclusion The specific agonist of RXR, 9-cisRA could effectively inhibit the differentiation of THP-1 human monocyte induced by PMA.