离心机训练对大鼠不同组织mRNA表达的影响

目的分析 5个与离心机训练有关的基因在大鼠各组织的表达水平及其与训练时间的关系。方法分别提取离心机训练不同时间组大鼠心、脑、肾、肺和肠 5种组织的mRNA并进行量化 ,采用狭线杂交方法 ,用Dig 1 1 dUTP标记 5个用抑制性消减杂交筛选离心机训练大鼠获得的基因片段作探针 ,杂交结果通过光密度扫描半定量后 ,进行统计学处理。结果未知基因CH1 5 7的表达水平在训练 6d时大鼠心脏和脑组织明显下降 (P <0 .0 5 ) ,训练 1 2d时有所回升 ;未知基因CH2 4 4的表达水平在大鼠心、脑、肠组织在训练 6d、1 2d时均下调 ,但其表达量个体差异表现突出 ,不呈现明显的统计学意义 ;神经元一氧化氮合酶抑制剂 (PIN)基因 ,随着离心机训练时间的延长 ,在大鼠心脏和肾脏组织中的表达水平明显上调(P <0 .0 5 ) ;细胞色素b (Cytb)基因在大鼠心脏、脑组织中表达水平随着训练时间增长而明显上调 (P<0 .0 1 ) ,在肾、肺和肠组织中的表达在训练 6d时明显升高 (P <0 .0 5 ) ,训练 1 2d时有所恢复 ;延长因子 1 α(EF1 α)基因在大鼠脑组织中表达水平随着训练时间增长而明显上调 ,在肠组织中的表达在训练 6d时明显升高 (P <0 .0 5 ) ,训练 1 2d时恢复到训练前水平。结论 5个基因在离心机训练大鼠不同组织中的表达水平存在差异 ,

Effects of Centrifuge Training on mRNA Expression in Different Rat Tissues

Objective: To demonstrate the tissue specific expression of five rat genes related to centrifuge training and the relationship between expression change and duration of training. Method: mRNA was extracted from hearts, brains, kidneys, lungs and intestines of rats after different durations of training. Five genes obtained by SSH from centrifuge-trained rats were labeled by Dig-11-dUTP as probes. The expression levels were measured by thin-line hybridization. Optical density (OD) values were obtained by scanning and treated with statistics. Result: As compared with control group, the new gene CH157 expression decreased in hearts and brains of rats trained for 6 d (P<0.05), but returned to control levels after 12 d training; the new gene CH244 expression depressed in rat hearts, brains, and intestines after 6 d or 12 d training, but it was not statistically significant owing to large individual differences; gene expression of protein inhibitor of neuronal nitric oxide syntheses (PIN) increased gradually and significantly in rat hearts (P<0.01 after 12 d training) and kidneys (P<0.05 after 12 d training); Cyt b gene expression in rat hearts and brains increased increasingly (P<0.01 after 12 d training), however, it increased obviously (P<0.05) in kidneys, lungs and intestines of rats trained for 6 d, while it returned back a little in rats trained for 12 d; enlongation factor 1-alpha expression levels in intestines of rats trained for 6 d increased significantly (P<0.05), but it became no significant in rats trained for 12 d, while the expression in brains increased gradually (P<0.01). Conclusion: It suggested that the change in gene expression in several tissues of rats caused by centrifuge training contribute in enhancing +Gz tolerance.