| Fgl2凝血酶原酶在小鼠免疫器官中的表达 |
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| 引用本文： | 郭国宁,朱国宴,尚永军,文亮,徐世伟. Fgl2凝血酶原酶在小鼠免疫器官中的表达[J]. 免疫学杂志, 2010, 26(5) |
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| 作者姓名： | 郭国宁 朱国宴 尚永军 文亮 徐世伟 |
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| 作者单位： | 第三军医大学附属西南医院急诊科,重庆,400038;重庆江陵医院妇产科,400038;内蒙古赤峰医学院第一附属医院骨科,024000 |
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| 基金项目： | 国家自然科学基金(30700855及60971117)资助 |
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| 摘 要： | 目的探讨小鼠免疫器官胸腺、脾脏及淋巴结组织中Fgl2凝血酶原酶基因转录及蛋白表达水平。方法使用RT-PCR分析Fgl2凝血酶原酶基因转录水平;免疫组化检测淋巴器官标本Fgl2凝血酶原酶表达;双色免疫荧光分析Fgl2凝血酶原酶与Foxp3表达的关系。结果RT-PCR结果证实正常的胸腺、脾脏及淋巴结有Fgl2凝血酶原酶基因转录。免疫组化的结果则表明,这些淋巴器官中有大量的Fgl2凝血酶原酶表达。免疫荧光分析结果显示胸腺及脾脏内的Fgl2~+细胞为Foxp3~-细胞。结论免疫器官内Fgl2凝血酶原酶的表达可能参与了T细胞发育及功能。此外,Fgl2不直接参与Foxp3~+调节性T细胞的抑制功能。
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| 关 键 词： | Fgl2 Foxp3 免疫器官 免疫组化 |
Expression of Fgl2 in mouse immune organs |
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| GUO Guoning,ZHU Guoyan,SHANG Yongjun,WEN Liang,XU Shiwei Emergency Department,Southwest Hospital,Third Military Medical University,Chongqing ,China. Expression of Fgl2 in mouse immune organs[J]. Immunological Journal, 2010, 26(5) |
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| Authors: | GUO Guoning ZHU Guoyan SHANG Yongjun WEN Liang XU Shiwei Emergency Department Southwest Hospital Third Military Medical University Chongqing China |
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| Affiliation: | GUO Guoning,ZHU Guoyan,SHANG Yongjun,WEN Liang,XU Shiwei Emergency Department,Southwest Hospital,Third Military Medical University,Chongqing 400038,China |
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| Abstract: | We investigated Fgl2 gene transcription and protein expression in mouse immune organs,including thymus,spleen, and lymph nodes,in the aim of benefiting further studying of Fgl2 immunoregulation function.Positive Fgl2 gene transcription in these immune organs was confirmed by RT-PCR.While immunohistochemical analysis revealed that Fgl2 specifically express on cytoplasm of some T cells within these organs.Moreover,double staining further confirmed that lots of Fgl2 positive cells are Foxp3 negative cells.Expr... |
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| Keywords: | Fgl2 Foxp3 Immune organs Immunohistochemistry |
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