Visceral and Somatic Hypersensitivity in TNBS-Induced Colitis in Rats |
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| Authors: | QiQi Zhou Donald D Price Robert M Caudle G Nicholas Verne |
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| Institution: | (1) Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, University of Florida College of Medicine, 1600 SW Archer Road, HD 602, Gainesville, FL 32610-0214, USA;(2) Department of Oral and Maxillofacial Surgery, University of Florida Colleges of Medicine and Dentistry, Gainesville, FL, USA;(3) Department of Neuroscience, University of Florida Colleges of Medicine and Dentistry, Gainesville, FL, USA;(4) North Florida/South Georgia VA Medical System, Gainesville, FL, USA |
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| Abstract: | Inflammation of visceral structures in rats has been shown to produce visceral/somatic hyperalgesia. Our objectives were to
determine if trinitrobenzene sulfonic acid (TNBS) induced colitis in rats leads to visceral/somatic hypersensitivity. Male
Sprague-Dawley rats (200–250 g) were treated with 20 mg of TNBS in 50% ethanol (n = 40) or an equivalent volume of ethanol (n = 40) or saline (n = 25) via the colon. Colonic distension, Von Frey, Hargreaves, and tail reflex tests were used to evaluate for visceral,
mechanical, and thermal sensitivity. The rats demonstrated visceral hypersensitivity at 2–28 days following TNBS administration
(P < 0.0001). The ethanol-treated rats also demonstrated visceral hypersensitivity that resolved after day 14. TNBS-treated
rats demonstrated somatic hypersensitivity at days 14–28 (P < 0.0001) in response to somatic stimuli of the hind paw. TNBS colitis is associated with visceral and somatic hypersensitivity
in areas of somatotopic overlap. This model of colitis should allow further investigation into the mechanisms of visceral
and somatic hypersensitivity. |
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| Keywords: | Trinitrobenzene sulfonic acid induced colitis Visceral hypersensitivity Somatic hypersensitivity Viscerosomatic convergence Irritable bowel syndrome |
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