| 结核杆菌H37Rv株重组PstS1蛋白的表达纯化及其免疫反应性分析 |
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| 引用本文： | 石君帆,宋广忠,泮结超,漏磊君,李召东. 结核杆菌H37Rv株重组PstS1蛋白的表达纯化及其免疫反应性分析[J]. 中国预防医学杂志, 2011, 0(4): 307-310 |
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| 作者姓名： | 石君帆 宋广忠 泮结超 漏磊君 李召东 |
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| 作者单位： | 浙江省医学科学院;杭州市红十字会医院; |
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| 基金项目： | 浙江省科技厅专项公益技术攻关项目资助(2007F10033); 浙江省医学科学院青年基金项目资助(A30810Q) |
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| 摘 要： | 目的:表达、纯化结核杆菌H37Rv株重组Psts1(简称rPstS1)蛋白,为结核病血清学诊断价值研究及亚单位疫苗研制提供物质基础.方法:重组质粒pET15b-Pstsl转化大肠埃希菌表达菌株BL21(DE3)plysE,IPTG诱导rPstSI蛋白表达.经SDS-PAGE电泳和Western blot鉴定后,优化表达...
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| 关 键 词： | Psts1 亲和层析 亚细胞定位 结核 |
Study on expression,purification and immunoreactivity of mycobacteriuml tuberculosis recombinant PstS1 |
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| SHI Jun-fan,SONG Guang-zhong,PAN Jie-chao,LOU Lei-jun,LI Zhao-dong.Zhejiang Academy of Medical Sciences,Hangzhou ,China. Study on expression,purification and immunoreactivity of mycobacteriuml tuberculosis recombinant PstS1[J]. China Preventive Medicine, 2011, 0(4): 307-310 |
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| Authors: | SHI Jun-fan SONG Guang-zhong PAN Jie-chao LOU Lei-jun LI Zhao-dong.Zhejiang Academy of Medical Sciences Hangzhou China |
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| Affiliation: | SHI Jun-fan,SONG Guang-zhong,PAN Jie-chao,LOU Lei-jun,LI Zhao-dong.Zhejiang Academy of Medical Sciences,Hangzhou 310013,China |
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| Abstract: | Objective To provide the basis for subunit vaccine development and serodiagnosis of tuberculosis by expressing and purifying phosphate-specific transport system 1(PstS1) gene from mycobacterium tuberculosis in E.coli.Methods First of all,recombinant plasmid pET15b-PstS1 was transformed into E.coli BL21(DE3) plysE,and then the recombinant PstS1(rPstS1) protein was expressed in E.coli BL21(DE3) plysE by inducement of IPTG.After identification by SDS-PAGE electrophoresis and western blot,the expression conditi... |
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| Keywords: | Phosphate-specific transport system 1 Affinity chromatography Subcellular localization Tuberculosis |
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