| 高效液相色谱法测定蝎宝胶囊中阿魏酸的含量 |
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| 引用本文： | 李锦燊,梁陈方,杨春旭.高效液相色谱法测定蝎宝胶囊中阿魏酸的含量[J].时珍国医国药,2009,20(7). |
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| 作者姓名： | 李锦燊 梁陈方 杨春旭 |
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| 作者单位： | 广西医科大学附属第四医院,广西,柳州,545005 |
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| 基金项目： | 广西科技厅攻关基金，广西柳州市科技局科技攻关基金 |
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| 摘 要： | 目的 建立测定蝎宝胶囊中阿魏酸含量的高效液相色谱法.方法 采用VP-ODS(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-1%冰醋酸溶液 (25∶75) 为流动相,流速为1 ml/min,检测波长:313 nm.结果 阿魏酸在2.5～25 μg/ml浓度范围内,峰面积与浓度线性关系良好,相关系数r=0.999 8,加样平均回收率为99.43%,RSD=2.15%(n=6).结论 该法简便,准确,可用于蝎宝胶囊中阿魏酸的含量测定.
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| 关 键 词： | 蝎宝胶囊 阿魏酸 高效液相色谱法 |
Determination of Ferulic Acid in Xiebao Capsule by HPLC |
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| LI Jin-shen,LIANG Chen-fang,YANG Chun-xu.Determination of Ferulic Acid in Xiebao Capsule by HPLC[J].Lishizhen Medicine and Materia Medica Research,2009,20(7). |
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| Authors: | LI Jin-shen LIANG Chen-fang YANG Chun-xu |
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| Abstract: | Objective To establish an HPLC method for determination of Ferulic acid content in Xiebao capsule.Methods Samples were determined by RP-HPLC on a VP-ODS column(250 mm×4.6 mm,5 μm) with a mixture of acetonitrile-1% acetic acid (25:75) as mobile phase at the flow velocity of 1 ml/min and UV detection at 320nm.Results The calibration curves of Ferulic acid was linear from 2.5 to 25μg/ml (r=0.999 8,n=6),the average recovery and RSD were 99.43% and 2.15% (n=6) respectively.Conclusion The method is simple and accurate.It can be used for determination of Ferulic acid content in Xiebao capsule. |
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| Keywords: | Xiebao capsule Ferulic acid HPLC |
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