聚氰基丙烯酸正丁酯纳米粒的生物相容性研究

目的 对药用载体聚氰基丙烯酸正丁酯纳米粒的生物相容性进行系统性评价。方法 体外采用MTT法进行细胞增殖测定、溶血试验,体内采用皮下和肌肉内埋植载体的炎症反应试验。结果 该载体对细胞属无毒级,无溶血性,在动物体内埋植3个月后载体降解,周围组织无明显炎症反应。结论 所制备的聚氰基丙烯酸正丁酯纳米粒载体具有良好的生物相容性。

Study of biocompatibility of polybutylcyanoacrylate nanoparticles

Objective To evaluate the biocompatibility of polybutylcyanoacrylate nanoparticles (PBCA-NP) manufactured by emulsion polymerization technique. Methods MTT assay was performed for evaluation of the cytotoxicity of PBCA-NP (e.g. cell proliferation and hemolytic test) experiments of long-term subcutaneous implantation and implantation in the muscular tissues of PBCA-NP was conducted in rabbits to observe the inflammatory reactions due to the implantation. Results No obvious cytotoxicity or hemolytic reactions were observed (with the hemolytic rate at different concentrations of PBCA-NP<5%). Three months after PBCA-NP implantation, obvious lymphocyte infiltration was not observed in the tissues around the implants which underwent gradual degradation. Conclusion PBCA-NP possesses good biocompatibility.