柯萨奇病毒B3感染人宫颈癌细胞株HeLa细胞后Bad mRNA表达的意义

目的探讨柯萨奇病毒B3(CVB3)诱导的Bad mRNA表达的意义。 方法体外培养的HeLa细胞通过CVB3感染建立模型，通过倒置显微镜下观察CVB3感染HeLa细胞后在不同时间点(3 h、6 h、9 h、12 h、24 h)细胞形态学变化，采用反转录聚合酶链式反应(RT-PCR)检测凋亡相关基因Bad mRNA的表达。采用SPSS 16.0统计软件进行统计分析，两组Bad mRNA的表达水平采用均数±标准差表示，显著性比较采用t检验。 结果CVB3感染HeLa细胞后对照组各时间点无形态上的变化；病毒组随着感染时间的延长，逐渐出现细胞病变效应，以感染24 h后最明显。对照组各时间点Bad mRNA的表达量比较差异无统计学意义(P>0.05)，病毒组各时间点Bad mRNA的表达量比较差异无统计学意义(P>0.05)，但感染24 h后病毒组Bad mRNA(0.61±0.06) s]表达低于对照组(0.76±0.06) s]，差异有统计学意义(t＝－3.445，P<0.05)，而3 h、6 h、9 h、12 h后病毒组和对照组Bad mRNA表达量的比较差异无统计学意义(t＝－0.97，－0.43，－3.9，－0.97；P>0.05)。 结论促凋亡基因Bad mRNA在CVB3病毒感染的HeLa细胞中发挥促凋亡作用。

The expression of Bad mRNA in Hela cells infected by coxsackievirus B3

ObjectiveTo investigate the significance of Bad mRNA induced by coxsackievirus B3 (CVB3). MethodsAfter HeLa cells infected by CVB3, the cellular morphology at different times of infection was observed under the inverted microscope, and Bad mRNA expression was determined by RT-PCR.The difference of Bad mRNA expression was compared with t-test. ResultsThe cellular morphology in control group was not obviously changed at different time points, but the cytopathic effect (CPE) in viral groups was significantly gradually changed at the infection time, which was most obvious at 24 h. At the meantime, the expression of Bad mRNA in control group was not significantly different at different infection time points(P>0.05), which was the same in the viral groups.But Bad mRNA expression in CVB3 group(0.61±0.06) was decreased compared with the control (0.76±0.06)(t=-3.445, P<0.05) at 24 h. However, there was no significance at other infection points such as 3h, 6h, 9h and 12h. ConclusionsAs the pro-apoptotic gene of Bad mRNA, plays an important role in the CVB3 infection which induces the apoptosis of Hela cells.