miR-34c对II型子宫内膜癌HEC-1-B细胞生长和凋亡的影响

目的 ：研究miR-34c对Ⅱ型子宫内膜癌HEC-1-B细胞的生长及凋亡的影响。方法：用hsa-miR-34c mimics转染HEC-1-B细胞。流式细胞技术测定细胞转染率，实时荧光定量PCR验证转染后miR-34c的表达。CCK-8检测细胞增殖能力的改变。细胞克隆形成实验观察miR-34c对细胞生长的长期抑制。流式细胞技术测细胞凋亡。结果：细胞转染率为81.16%。相对于对照组，转染后实验组miR-34c表达明显增加，细胞增殖、克隆形成能力减弱，凋亡增加（P＜0.05）。结论：miR-34c能明显抑制Ⅱ型子宫内膜癌细胞的增殖，促进细胞凋亡，是Ⅱ型子宫内膜癌的潜在抑癌基因。

The influence of miR-34c on growth and apoptosis of type II endometrial carcinoma cell line HEC-1-B

Objective: To investigate the effect miR-34c on the growth and apoptosis of type II endometrial carcinoma cells (HEC-1-B cell line). Methods: Up-regulated the expression of miR-34c by transfecting cells with hsa-miR-34c mimics, the transfection efficiency was detected by flow cytometry and further verified by quantitative real-time PCR (qRT-PCR). Cell proliferation assay by CCK-8 and colony formation assay applied to demonstrate that miR-34c could inhibit the growth of HEC-1-B cells. Cells apoptosis assay was analyzed by flow cytometry. Results: The transfection efficiency was 81.16%. The overexpression of miR-34c (detected by qRT-PCR) could inhibit cell proliferation, colony formation and promote cells apoptosis (P<0.05). Conclusion: MiR-34c can inhibit the growth and promote apoptosis of HEC-1-B cells significantly, and function is a potential tumor suppressor in type II endometrial carcinoma.