莫诺苷对局灶性脑缺血再灌注大鼠海马CyclinD1及Cdk6的影响

目的: 观察莫诺苷对局灶性脑缺血再灌注大鼠细胞周期蛋白的影响。方法: 用线栓法制备大鼠局灶性脑缺血再灌注模型。将15只Sprague-Dawley大鼠随机分为假手术组，模型组，莫诺苷低、中、高剂量组(30mg·kg-1、90 mg·kg-1、270 mg·kg-1)。利用免疫组化方法检测大鼠患侧海马CyclinD1、Cdk6表达。结果: 与假手术组相比，模型组CyclinD1及Cdk6表达显著增加；但给予莫诺苷治疗后，与模型组相比，莫诺苷中、高剂量能显著降低大鼠CyclinD1及Cdk6的表达。结论: 莫诺苷能通过降低脑缺血再灌注后CyclinD1及Cdk6的表达而发挥神经保护作用。

Effects of Morroniside on the expression of CyclinD1 and Cdk6 in Hippocampus of Rats with Focal Cerebral Ischemia/Reperfusion Injury

Objective: To explore the effects of morroniside on key cell-cycle regulator proteins in a rat model of focal cerebral ischemia-reperfusion. Methods: After the model of middle cerebral artery occlusion (MCAO) was established, 15 Sprague-Dawley rats were randomly assigned into sham group, model group, morroniside low dose (30 mg·kg-1) group, morroniside middle dose (90 mg·kg-1) group and morroniside high dose (270 mg·kg-1) group. Immunohistochemistry staining was used to measure the expression of CyclinD1 and Cdk6 in ischemic ipsilateral hippocampus. Results: Compared with sham group, the expression of CyclinD1 and Cdk6 were significantly increased in vehicle-treated ischemic-reperfusion rats. However, the expression of CyclinD1 and Cdk6 was significantly reduced by treatment of morroniside at doses of 90 mg·kg-1 and 270 mg·kg-1 after ischemia-reperfusion. Conclusion: Morroniside might play a role of neuroprotection by downregulating the expression of CyclinD1 and Cdk6 after cerebral ischemia-reperfusion.