白三烯D4在体外对A549肺泡上皮细胞增殖及迁移的影响

目的：观察炎症介质白三烯D4（LTD4）对人肺泡上皮细胞株A549细胞增殖及迁移的影响。方法：以免疫荧光染色鉴定A549细胞上半胱氨酰白三烯（CysLT）受体的表达；以不同浓度（001～100 nmol/L）的CysLT受体激动剂LTD4处理A549细胞不同时间后，以MTT还原法检测细胞活性反映细胞增殖，以改良的划痕法观察细胞迁移的变化。结果：A549细胞表达CysLT1受体及CysLT2受体。001～100 nmol/L LTD4处理A549细胞24～72 h，细胞增殖无明显变化（均P>005）；100 nmol/L LTD4处理A549细胞24、48、72 h后，细胞活性分别为不加LTD4处理的A549细胞的（103.00±446）%、（107.00±945）%、（105.00±902）%，差异均无统计学意义（均P>005）。虽然001～100 nmol/L LTD4处理的第1个24 h内A549细胞的修复速率远高于第2个及第3个24 h内的细胞修复速率，但同一时间段组间差异无统计学意义（均P>005）。100 nmol/L 的LTD4处理A549细胞24、48、72 h后，细胞迁移率分别为不加LTD4处理的A549细胞的115、121、106倍（均P>005），说明LTD4处理后细胞迁移亦无明显变化。结论：在体外给予001～100 nmol/L LTD4处理72 h，不影响A549细胞的增殖及迁移。

Effects of leukotriene D4 on proliferation and migration of lung epithelial A549 cells in vitro

Objective： To investigate the effects of cysteinyl leukotriene （CysLT） receptor agonist leukotriene D4 （LTD4） on proliferation and migration in lung epithelial A549 cells. Methods： The expression of CysLT1 receptor and CysLT2 receptor was determined by immunofluoresence staining in A549 cells. A549 cells were treated with LTD4 （001 100 nmol/L） for 24 72 h. Cell viability was detected by MTT reduction assay. Cell migration was determined by modified scratch and healing model. Results： In A549 cells， CysLT1 receptor and CysLT2 receptor were mainly expressed in the cytoplasm， membrane and few in the nuclei. The treatment of LTD4 （001 100 nmol/L） for 24 72 h caused no effect on cell viability （Ps>005）; when A549 cells were treated with 100 nmol/L LTD4 for 24， 48 and 72 h the cell viability was （103.00±446）%，（107.00±945）% and （105.00±902）% of control， respectively （Ps>005）. The migration rate of A549 cells after scratching during the first 24 h was markedly greater than that during the second and third 24 h in the same concentration groups; however， no significant difference in migration rate was noticed when the cells were treated with different concentrations of LTD4 （001 100 nmol/L）（Ps>005）. The migration of A549 cells was 115 fold， 121 fold and 106 fold of that of control when the cells were treated with 100 nmol/L LTD4 for 24， 48 and 72 h， respectively （Ps>005）. Conclusion： The proliferation and migration of A549 cells are not changed when treated with 001 100 nmol LTD4 for up to 72h.