痛风患者血清中IL-37、IL-1β及IL-18水平检测及其相关性研究#br#

目的：通过检测痛风患者不同阶段血清中白细胞介素（IL）-37、IL-1β及IL-18水平，探讨IL-37在痛风发病中的作用。方法：采用酶联免疫吸附（ELISA）法测定20例初发痛风患者（痛风组，急性发作期和完全缓解期）、20例高尿酸血症患者（高尿酸血症组）及20例健康志愿者（健康对照组）血清中IL-37、IL-1β及IL-18的水平，比较痛风组患者急性发作期和完全缓解期各指标的表达差异，以及痛风组患者急性发作期及完全缓解期与其他两组各指标的表达差异；同时分析急性发作期患者IL-37水平与血沉（ESR）、C反应蛋白（CRP）、IL-1β和IL-18水平的相关性。结果：痛风组患者急性发作期的IL-1β和IL-18水平均显著高于完全缓解期，差异具有统计学意义（P＜0.05）；而IL-37的水平仅略高于完全缓解期，差异无统计学意义（P＞0.05）。痛风组患者急性发作期IL-37、IL-1β及IL-18水平均高于高尿酸血症组和健康对照组，差异均具有统计学意义（P＜0.05）；而完全缓解期仅IL-37水平高于高尿酸血症组和健康对照组（P＜0.05）。痛风组患者急性发作期的IL-37水平与其ESR、CRP、IL-1β、IL-18水平呈正相关（均P＜0.01）。结论：IL-37可能作为一个抑炎因子参与了痛风的炎症反应过程。

Detection and correlation study of IL-37, IL-1β and IL-18 in gout patients

Objective: To determine levels of interleukin-37 (IL-37), IL-1β and IL-18 in serum of patients with gout at difference stages, and to explore the role of IL-37 in the pathogenesis of gout. Methods: The levels of IL-37, IL-1β and IL-18 in 20 patients with gout, 20 patients with hyperuricemia and 20 healthy controls were detected wtih ELISA, the changes and correlations in different groups were analyzed. Results: Gout group in acute attack stage IL-1β and IL-18 levels was significantly higher than those in complete remission period (P<0.05), while the IL-37 level was only slightly higher than that of complete remission period (P>0.05). IL-37, IL-1β and IL-18 in gout group at acute attack stage were significantly higher than that in the hyperuricemia group and healthy control group (P<0.05). The higher expressions of IL-37 in complete remission period were detected and compared with the hyperuricemia group and healthy control group, whereas there was no difference in IL-1β and IL-18 levels (P>0.05). The level of serum IL-37 in gout group at acute attack stage, was positively correlated with ESR, CRP, IL-1β and IL-18 (P<0.01). Conclusion: IL-37 as an inhibiting inflammation factor probably takes part in the process of inflammatory respense of gout.