| HPLC法测定注射用地西他滨的含量 |
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| 引用本文： | 袁玉,朱梅红. HPLC法测定注射用地西他滨的含量[J]. 西北药学杂志, 2012, 27(6): 540-542 |
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| 作者姓名： | 袁玉 朱梅红 |
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| 作者单位： | 袁玉 (江苏奥赛康药业有限公司,南京,211112) ； 朱梅红 (江苏奥赛康药业有限公司,南京,211112) ； |
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| 摘 要： | 目的建立测定注射用地西他滨含量的HPLC法。方法采用Kromasil C18柱(250mm×4.6mm,5μm);流动相为0.01mol.L-1 K2HPO4(用1mol.L-1 HCl调节pH值为6.0);流速为1mL.min-1;柱温为25℃±2℃;检测波长为220nm。结果地西他滨进样量在57.92～1 158.3ng范围内与峰面积呈良好的线性关系(r=0.999 8),平均加样回收率为100.0%(n=9),RSD为0.5%。结论该法操作简便、准确可靠,可作为注射用地西他滨含量测定的方法。
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| 关 键 词： | 高效液相色谱法 地西他滨 含量测定 |
Determination of Decitabine for Injection by HPLC |
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| YUAN Yu,ZHU Meihong. Determination of Decitabine for Injection by HPLC[J]. Northwest Pharmaceutical Journal, 2012, 27(6): 540-542 |
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| Authors: | YUAN Yu ZHU Meihong |
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| Affiliation: | (Jiangsu Aosaikang Pharmaceutical Co.Ltd,Nanjing 211112,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of Decitabine for Injection.Methods The test was carried out on a Kromasil C18 column（250 mm×4.6 mm,5 μm） with 0.01 mol·L-1 K2HPO4（pH value 6.0 adjusted by 1 mol·L-1 HCl） as the mobile phase,the flow rate was 1 mL·min-1,the column temperature was 25 ℃±2 ℃,and the detection wavelength was 220 nm.Results Within the range of 57.92-1 158.3 ng,there was a good linear correlation（r=0.999 8）.The average recovery was 100.0%（n=9）,and RSD was 0.5%.Conclusion The method is simple,accurate,and can be used for the determination of Decitabine for Injection. |
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| Keywords: | HPLC decitabine assay |
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