| HPLC法同时测定黄芪中4种成分的含量 |
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| 引用本文： | 杨范莉,宋立强. HPLC法同时测定黄芪中4种成分的含量[J]. 西北药学杂志, 2012, 27(6): 526-527 |
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| 作者姓名： | 杨范莉 宋立强 |
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| 作者单位： | 杨范莉 (西安交通大学医学部药学系,西安,710061) ； 宋立强 (第四军医大学附属西京医院,西安,710032) ； |
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| 摘 要： | 目的建立同时测定黄芪饮片中毛蕊异黄酮苷、刺芒柄花苷、毛瑞异黄酮和刺芒柄花素的方法。方法 HPLC-UV测定,色谱条件为Shimadzu VP-ODS C18分析柱(250mm×4.6mm,5μm);柱温30℃;流动相为A0.5mL.L-1甲酸水溶液,B乙腈,梯度洗脱;流速1.0mL.min-1;检测波长254nm。结果该方法能够同时对黄芪中4个成分进行含量测定,精密度、稳定性、回收率均能够满足含量测定要求。结论该方法简便、灵敏,结果可靠,可用于黄芪饮片的质量控制。
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| 关 键 词： | HPLC 黄芪 化学成分 |
Simultaneous determination of the four components of Radix Astragali by HPLC |
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| YANG Fanli,SONG Liqiang. Simultaneous determination of the four components of Radix Astragali by HPLC[J]. Northwest Pharmaceutical Journal, 2012, 27(6): 526-527 |
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| Authors: | YANG Fanli SONG Liqiang |
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| Affiliation: | 1.College of Pharmacy,School of Medicine,Xi’an Jiaotong University,Xi’an,710061 China;2.Xijing Hospital,the Fourth Military Medical University,Xi’an 710032,China) |
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| Abstract: | Objective To establish an HPLC method for the simultaneous determination of the 4 components in Radix Astragali.Methods HPLC analysis was performed on a Shimadzu VP-ODS C18 column（250 mm×4.6 mm,5 μm）.The column temperature was 30 ℃,the mobile phases were 0.5 mL·L-1 formic acid solution and acetonitrile,gradient elution was carried out,and the flow rate was 1.0 mL·min-1.Results The established HPLC method was successfully utilized to determine the 4 constituents with satisfactory precision,stability and recovery.Conclusion The method was simple,sensitive and reliable,and it could be used for the quality control of Radix Astragali. |
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| Keywords: | HPLC Radix Astragali components |
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